葡萄糖诱导构巢曲霉中异柠檬酸裂解酶的失活。

Glucose-induced inactivation of isocitrate lyase in Aspergillus nidulans.

作者信息

De Lucas J R, Valenciano S, Laborda F, Turner G

机构信息

Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Alcalá de Henares, Madrid, Spain.

出版信息

Arch Microbiol. 1994;162(6):409-13. doi: 10.1007/BF00282105.

DOI:10.1007/BF00282105

PMID:7872837

Abstract

The existence of a second mechanism of catabolite control of isocitrate lyase of Aspergillus nidulans, in addition to the carbon catabolite repression phenomenon recently reported was analysed. Isocitrate lyase was rapidly and specifically inactivated by glucose. The inactivation was irreversible at all stages in the presence of cycloheximide, showing that reactivation depends on de novo protein synthesis. In addition, analysis of glucose-induced inactivation of isocitrate lyase in a creAd-30 strain showed that the creA gene is not involved in this process.

摘要

除了最近报道的碳分解代谢物阻遏现象外，还分析了构巢曲霉异柠檬酸裂解酶分解代谢物控制的第二种机制的存在情况。异柠檬酸裂解酶被葡萄糖迅速且特异性地失活。在环己酰亚胺存在的所有阶段，这种失活都是不可逆的，这表明重新激活依赖于从头合成蛋白质。此外，对creAd-30菌株中葡萄糖诱导的异柠檬酸裂解酶失活的分析表明，creA基因不参与此过程。