Kondo N, Takao A, Tomatsu S, Shimozawa N, Suzuki Y, Ogawa T, Iwata H, Orii T
Department of Pediatrics, Gifu University School of Medicine, Gifu, Japan.
J Investig Allergol Clin Immunol. 1994 Sep-Oct;4(5):255-7.
In this study, the mechanisms for suppression of immunoglobulin production by lymphocytes induced by diphenylhydantoin (DPH) were investigated using peripheral blood mononuclear cells from one patient 3 months after discontinuation of DPH, when IgA deficiency was improved. Immunoglobulin production in PBMCs was dose-dependently suppressed by DPH and the suppressive effect of DPH on IgA production tended to be greater than the effect on IgM or IgG production. Moreover, cell separation experiments indicated that DPH suppresses immunoglobulin production by PBMCs through a suppression of B cell, but not T cell, function. The finding that the suppressive effect of DPH on IgA production tended to be greater than the effect on IgM or IgG production might be related to the fact that the alpha-chain gene is located in the 3' end of the gene coding the constant regions of human heavy chains.
在本研究中，使用1例患者停用苯妥英（DPH）3个月后（此时IgA缺乏有所改善）的外周血单个核细胞，研究了DPH诱导淋巴细胞抑制免疫球蛋白产生的机制。PBMCs中的免疫球蛋白产生受到DPH的剂量依赖性抑制，且DPH对IgA产生的抑制作用往往大于对IgM或IgG产生的抑制作用。此外，细胞分离实验表明，DPH通过抑制B细胞而非T细胞功能来抑制PBMCs产生免疫球蛋白。DPH对IgA产生的抑制作用往往大于对IgM或IgG产生的抑制作用这一发现，可能与α链基因位于编码人重链恒定区的基因3'端这一事实有关。
