Cariño F A, Koener J F, Plapp F W, Feyereisen R
Department of Chemistry, Ateneo de Manila University, Loyola Heights, Quezon City, Philippines.
Insect Biochem Mol Biol. 1994 Apr;24(4):411-8. doi: 10.1016/0965-1748(94)90034-5.
Messenger RNA levels of the cytochrome P450 gene CYP6A1 were measured in the insecticide resistant Diazinon-R 'Rutgers' strain and in the susceptible strain sbo of the house fly with a cloned cDNA probe. The constitutive expression of the CYP6A1 gene was at least 10 times higher in the Rutgers strain than in the sbo strain. In both strains, CYP6A1 was inducible by phenobarbital treatment of the flies. Analysis of genomic DNA from the two strains indicated that there was no amplification of the CYP6A1 gene in the Rutgers strain. A developmental analysis revealed that CYP6A1 is expressed larvae and adults, and very low levels of CYP6A1 mRNA were detected in eggs and pupae. The constitutive overexpression of CYP6A1 in the Rutgers strain was observed in both larvae and adults. Crosses between the Rutgers strain and the multiply marked sbo strain revealed that the high constitutive expression of CYP6A1 in the Rutgers strain is controlled by one or more loci located on chromosome II.
用克隆的 cDNA 探针测定了抗杀虫剂的二嗪农 -R“罗格斯”品系和家蝇敏感品系 sbo 中细胞色素 P450 基因 CYP6A1 的信使 RNA 水平。CYP6A1 基因的组成型表达在罗格斯品系中比在 sbo 品系中至少高 10 倍。在两个品系中，用苯巴比妥处理果蝇均可诱导 CYP6A1。对两个品系的基因组 DNA 分析表明，罗格斯品系中 CYP6A1 基因没有扩增。发育分析显示，CYP6A1 在幼虫和成虫中表达，在卵和蛹中检测到极低水平的 CYP6A1 mRNA。在罗格斯品系的幼虫和成虫中均观察到 CYP6A1 的组成型过表达。罗格斯品系与多重标记的 sbo 品系之间的杂交表明，罗格斯品系中 CYP6A1 的高组成型表达受位于二号染色体上的一个或多个基因座控制。
