Nimri L F, Hijazi S S
Department of Biological Sciences, School of Medicine, Jordan University of Science and Technology, Irbed.
J Clin Gastroenterol. 1994 Dec;19(4):288-91. doi: 10.1097/00004836-199412000-00005.
In this case-control study, we investigated the role of Cryptosporidium in gastroenteritis in children < 6 years old. Six hundred fresh stool specimens were examined for various pathogenic parasites, bacteria, and rotaviruses. Wet-mount preparations, formaline-ether concentrations, and Sheather's floatation techniques were used to recover the parasite oocysts. Permanent stained slides using acid-fast stain and trichrome stains were prepared. Of 300 children with gastroenteritis symptoms, 20 (6.7%) had Cryptosporidium oocysts; seven of the 20 had concomitant infections so they were excluded from the counts. This infection rate is significantly different (Z = 2; p < 0.05) from that found in the control group (1.7%) of children who reported no symptoms. The most frequent symptoms reported beside diarrhea were abdominal pain, cramps, anorexia, nausea, vomiting, and fatigue. Contaminated drinking water is suspected to be the source of infection; other possible factors are discussed.
在这项病例对照研究中，我们调查了隐孢子虫在6岁以下儿童肠胃炎中的作用。对600份新鲜粪便标本进行了各种致病性寄生虫、细菌和轮状病毒检测。采用湿片法、甲醛乙醚浓缩法和Sheather漂浮法来回收寄生虫卵囊。制备了使用抗酸染色和三色染色的永久染色玻片。在300名有肠胃炎症状的儿童中，20名（6.7%）有隐孢子虫卵囊；这20名中有7名有合并感染，因此在计数中被排除。该感染率与无症状儿童对照组（1.7%）的感染率有显著差异（Z = 2；p < 0.05）。除腹泻外报告的最常见症状是腹痛、痉挛、厌食、恶心、呕吐和疲劳。怀疑受污染的饮用水是感染源；还讨论了其他可能的因素。
