Nimri L F, Batchoun R
Department of Biological Sciences, Faculty of Science, Jordan University of Science and Technology, Irbed.
J Clin Microbiol. 1994 Apr;32(4):1040-2. doi: 10.1128/jcm.32.4.1040-1042.1994.
The project described here was conducted to study the prevalence of various parasites in elementary school children in northern Jordan. A single stool specimen was collected from each of 1,000 students in the 6- to 14-year-old age group. A questionnaire covering demographic information, health status, and other relevant information was filled out by one of the parents of each student. Fresh stool specimens were processed by using wet mount preparations, formalin-ether, and Sheather's sugar flotation techniques. Permanently stained slides were prepared by acid-fast, Giemsa, and trichrome staining. Cryptosporidium species was found in 40 specimens (4%); however, only 15 specimens had Cryptosporidium species alone, and these 15 specimens were from symptomatic children with diarrheic stools. The symptoms reported most often were abdominal pain, cramps, malaise, nausea, and headache. The number of cases of infection was higher in villages, where contact with animals was evident and where contaminated drinking water could have been a major source of the infections.
此处描述的项目旨在研究约旦北部小学生中各种寄生虫的流行情况。从1000名6至14岁年龄组的学生中每人采集了一份粪便样本。每位学生的一位家长填写了一份涵盖人口统计学信息、健康状况及其他相关信息的问卷。新鲜粪便样本采用湿片法、福尔马林-乙醚法和谢弗氏糖浮选技术进行处理。通过抗酸染色、吉姆萨染色和三色染色制备永久染色玻片。在40份样本（4%）中发现了隐孢子虫属；然而，仅有15份样本单独感染了隐孢子虫属，且这15份样本均来自有腹泻症状的儿童。最常报告的症状为腹痛、痉挛、不适、恶心和头痛。在村庄中感染病例数更高，在这些村庄中与动物接触明显，且受污染的饮用水可能是感染的主要来源。
