Tanaka A, Nishimura Y, Sakaki T, Fujita A, Shin-ike T
J Osaka Dent Univ. 1989 Oct;23(2):111-20.
Hydroxyapatite, lanthanum-containing hydroxyapatite, and lanthanum- and zirconium-containing hydroxyapatite discs were implanted subcutaneously in the backs of rats. The implanted discs and surrounding tissues were removed at 5 and 12 hours, and 1, 3, 7, 14, 30 and 60 days after implantation, and fixed in paraformaldehyde. Tissue samples were dehydrated and embedded in paraffin. Paraffin sections were cut and stained with hematoxylin and eosin, van Gieson's, and Masson's trichrome. The early tissue reaction was exudative due to fibrin deposition and infiltration by polymorphonuclear leukocytes and macrophages. The later reaction was characterized by the formation of fibrous tissue without inflammatory cell infiltration. The tissue reaction to all three types of material was identical by two months after implantation.
将羟基磷灰石、含镧羟基磷灰石以及含镧和锆的羟基磷灰石圆盘皮下植入大鼠背部。在植入后5小时、12小时、1天、3天、7天、14天、30天和60天取出植入的圆盘及周围组织，用多聚甲醛固定。组织样本脱水后用石蜡包埋。切取石蜡切片，并用苏木精和伊红、范吉森氏染色法以及马松三色染色法染色。早期组织反应为渗出性，这是由于纤维蛋白沉积以及多形核白细胞和巨噬细胞浸润所致。后期反应的特征是形成无炎症细胞浸润的纤维组织。植入两个月后，对所有三种材料的组织反应相同。
