Magez S, Lucas R, Darji A, Songa E B, Hamers R, De Baetselier P
Unit of Cellular Immunology, Institute of Molecular Biology, Sint-Genesius-Rode, Belgium.
Parasite Immunol. 1993 Nov;15(11):635-41. doi: 10.1111/j.1365-3024.1993.tb00577.x.
Soluble extracts from salivarian trypanosomes (Trypanosoma brucei brucei, T. evansi and T. congolense) were shown to be capable of inducing murine tumour necrosis factor (mTNF) secretion, both in vivo and in vitro, whereas the soluble extract of an intracellular trypanosome (T. cruzi) failed to do so. Furthermore, the role of mTNF during the initial phase of experimental infections with T. brucei was studied by treating infected mice with mTNF-inducing trypanosoma soluble extract and with neutralizing monoclonal anti-mTNF antibodies. Treatment of the infected animals with different doses of T. brucei soluble extract resulted in a lower first parasitaemia peak (low lysate dose) and in a longer survival time or in a nearly total inhibition of parasite development (high lysate dose). Cotreatment of the infected mice with both anti-mTNF antibodies and a high dose of soluble extract completely restored the parasite development in both trypanosusceptible C3H/He mice and trypanosubtolerant CBA Ca mice, indicating a protective role of mTNF during the parasitaemia. Collectively these results suggest a negative influence of mTNF on T. brucei development in vivo.
已证明，来自涎源性锥虫（布氏布氏锥虫、伊氏锥虫和刚果锥虫）的可溶性提取物在体内和体外均能诱导小鼠肿瘤坏死因子（mTNF）分泌，而细胞内锥虫（克氏锥虫）的可溶性提取物则不能。此外，通过用诱导mTNF的锥虫可溶性提取物和中和性单克隆抗mTNF抗体处理感染小鼠，研究了mTNF在布氏锥虫实验感染初始阶段的作用。用不同剂量的布氏锥虫可溶性提取物处理感染动物，导致第一个寄生虫血症峰值降低（低裂解物剂量），存活时间延长，或几乎完全抑制寄生虫发育（高裂解物剂量）。用抗mTNF抗体和高剂量可溶性提取物共同处理感染小鼠，在锥虫易感的C3H/He小鼠和锥虫耐受的CBA Ca小鼠中均完全恢复了寄生虫发育，表明mTNF在寄生虫血症期间具有保护作用。总体而言，这些结果表明mTNF对布氏锥虫在体内发育具有负面影响。
