Podstawka U
Zakładu Fizjopatologii Instytutu Hematologii i Transfuzjologii, Warszawie.
Rocz Panstw Zakl Hig. 1994;45(1-2):119-23.
The purpose of the study was to assess the phagocytic activity and bactericidal function of human peripheral blood neutrophils exposed to dichlorvos (DDVP) an organic phosphorus pesticide in vitro. It was found that the values of the phagocytosis index in neutrophil cultures incubated with DDVP in doses of 70 microM and 100 microM increased with increasing pesticide concentration and depended on incubation duration (1 h and 2 h). The proportion of non-phagocytizing neutrophils remained at a level similar to that in controls. The value of NBT reduction in neutrophils (indicating their bactericidal activity) after 1 hour of incubation with DDVP remained at the level of control values, but after 2 hours it decreased--significantly at DDVP concentration of 70 microM, and at DDVP concentration 100 microM p = 0.05. These results show that DDVP causes in neutrophils a selective stimulation of one step of phagocytosis connected with engulfing of particles, with simultaneous inhibition and/or damage to the mechanisms connected with aerobic killing of microorganisms.
本研究的目的是评估体外暴露于有机磷农药敌敌畏（DDVP）的人外周血中性粒细胞的吞噬活性和杀菌功能。研究发现，在含有70微摩尔和100微摩尔剂量DDVP的中性粒细胞培养物中，吞噬指数值随着农药浓度的增加而升高，并且取决于孵育时间（1小时和2小时）。未进行吞噬的中性粒细胞比例维持在与对照组相似的水平。与DDVP孵育1小时后，中性粒细胞的NBT还原值（表明其杀菌活性）维持在对照值水平，但孵育2小时后降低——在70微摩尔DDVP浓度下显著降低，在100微摩尔DDVP浓度下p = 0.05。这些结果表明，DDVP对中性粒细胞中与颗粒吞噬相关的吞噬作用的一个步骤有选择性刺激作用，同时对与需氧杀灭微生物相关的机制有抑制和/或损害作用。
