McCausland I P, Gavin J B, Herdson P B
Pathology. 1977 Apr;9(2):123-8. doi: 10.3109/00313027709085250.
The incorporation of intravenously injected dextran molecules (molecular weight 500,000 & 2,000,000) by the glomeruli of laboratory rats was studied by electron microscopy. In normal rats some dextran molecules were observed 12 minutes after injection in vesicles and invaginations of both endothelial and mesangial cells. Rats with nephrotoxic serum nephritis showed extensive separation of endothelial cells from the glomerular basement membrane. These subendothelial spaces contained irregular granular material, cytoplasmic extensions of mesangial cells and, in animals injected with dextran, dense accumulations of dextran molecules. There was no apparent difference in distribution of the two test samples of dextran. These findings demonstrated the involvement of both endothelial and mesangial cells in the glomerular localization of circulating material and indicated that immunologically inactive macromolecules can be incorporated into subendothelial deposits following immunological injury to the glomerulus.
通过电子显微镜研究了实验室大鼠肾小球对静脉注射的葡聚糖分子（分子量500,000和2,000,000）的摄取情况。在正常大鼠中，注射后12分钟可在内皮细胞和系膜细胞的小泡及内陷中观察到一些葡聚糖分子。患有肾毒性血清性肾炎的大鼠显示内皮细胞与肾小球基底膜广泛分离。这些内皮下间隙含有不规则颗粒物质、系膜细胞的细胞质延伸，并且在注射葡聚糖的动物中，有密集的葡聚糖分子聚集。两种葡聚糖测试样品的分布没有明显差异。这些发现证明了内皮细胞和系膜细胞都参与了循环物质在肾小球的定位，并表明免疫惰性大分子可在肾小球受到免疫损伤后掺入内皮下沉积物中。
