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[肠炎沙门氏菌菌株质粒DNA的筛选与限制性分析]

[The screening and restriction analysis of the plasmid DNA of Salmonella enteritidis strains].

作者信息

Riabchenko L E, Rashidov A M, Riapis L A

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1994 Sep-Oct(5):17-9.

PMID:7879474
Abstract

The screening and restriction analysis of plasmid DNA of 36 S. enteritidis strains, isolated in Moscow and Novomoskovsk (Tula Province) at the periods of outbreaks and sporadic diseases, have been made. The possibility of using plasmid screening as an additional method for the differentiation of S. enteritidis strains belonging to different sources of infection has been shown. The restriction analysis has confirmed the conservative character of the nucleotide sequence of the virulence plasmid with a molecular weight of 38 MD, which makes it difficult to use this method for epidemiological purposes.

摘要

对在莫斯科和新莫斯科斯克(图拉州)疫情爆发和散发病例期间分离出的36株肠炎沙门氏菌菌株的质粒DNA进行了筛选和限制性分析。结果表明,利用质粒筛选作为区分不同感染源的肠炎沙门氏菌菌株的附加方法是可行的。限制性分析证实了分子量为38 MD的毒力性质粒核苷酸序列的保守性,这使得该方法难以用于流行病学目的。

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