体外大肠杆菌核糖体依赖DNA合成蛋白质L12 。
DNA dependent synthesis of protein L12 from escherichia coli ribosomes, in vitro.
作者信息
Chu F, Kung H F, Caldwell P, Weissbach H, Brot N
出版信息
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3156-9. doi: 10.1073/pnas.73.9.3156.
Abstract
The in vitro synthesis of ribosomal protein L12 has been obtained in a coupled system with DNA extracted from the transducing phage lambdarifd18 as template. In addition, a second protein (molecular weight of 16,000) with immunological, chemical, and ribosome-binding characteristics similar to L12 is formed in this in vitro system. The synthesis of both proteins is depressed by one-half when guanosine-5'-diphosphate-3'-diphosphate is added to the reaction mixture.
摘要
以从转导噬菌体λrifd18中提取的DNA为模板，在偶联系统中实现了核糖体蛋白L12的体外合成。此外，在该体外系统中还形成了第二种蛋白质（分子量为16,000），其具有与L12相似的免疫学、化学和核糖体结合特性。当向反应混合物中加入5'-二磷酸鸟苷-3'-二磷酸时，这两种蛋白质的合成均减少一半。
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