Aardema M J, Crosby L L, Gibson D P, Kerckaert G A, LeBoeuf R A
Miami Valley Laboratories, Procter & Gamble Company, Cincinnati, OH 45253-8707, USA.
Cancer Genet Cytogenet. 1997 Jul 15;96(2):140-50. doi: 10.1016/s0165-4608(96)00301-9.
To gain a better understanding of the role of specific numerical and structural chromosome changes in the multistage process of transformation of Syrian hamster embryo (SHE) cells, we analyzed seven benzo(a)pyrene (BP)-induced immortal SHE cell lines, and one spontaneously immortalized cell line. In addition, we analyzed chromosome changes in early passage tumor-derived cell lines induced by injection of four immortalized cell lines into neonate hamsters. Of particular interest was the observation of a deletion in the short arm of chromosome 2 in four of the seven BP-immortalized cell lines. Other types of alterations in chromosome 2 were observed in two other cell lines. Loss of one copy of chromosome 16 was also observed in more than 90 to 100% of the cells in three of seven BP-immortalized cell lines. In contrast, the only chromosome alteration seen in the spontaneously immortalized cell line was a deletion in the short arm of chromosome 20. Genetic instability, as indicated by increased numerical or structural chromosome changes, was observed in all tumor-derived cell lines compared to the immortal cell line from which they originated. These results, along with previous reports in the literature, suggest that alterations in specific chromosomes, like chromosome 2, may be involved in transformation of SHE cells.
为了更好地理解特定的染色体数目和结构变化在叙利亚仓鼠胚胎（SHE）细胞转化的多阶段过程中的作用，我们分析了7个苯并(a)芘（BP）诱导的永生化SHE细胞系，以及1个自发永生化的细胞系。此外，我们还分析了将4个永生化细胞系注射到新生仓鼠体内诱导产生的早期传代肿瘤衍生细胞系中的染色体变化。特别值得关注的是，在7个BP永生化细胞系中的4个中观察到了2号染色体短臂的缺失。在另外两个细胞系中观察到了2号染色体的其他类型改变。在7个BP永生化细胞系中的3个中，超过90%至100%的细胞还观察到了16号染色体的单拷贝缺失。相比之下，在自发永生化细胞系中观察到的唯一染色体改变是20号染色体短臂的缺失。与它们起源的永生化细胞系相比，在所有肿瘤衍生细胞系中均观察到遗传不稳定性，表现为染色体数目或结构变化增加。这些结果，连同文献中先前的报道，表明特定染色体如2号染色体的改变可能参与了SHE细胞的转化。
