Oliveira L H, Frugulhetti I C, Passos M R, Cavalcanti S M, Fonseca M E
Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, RJ, Brasil.
Mem Inst Oswaldo Cruz. 1994 Apr-Jun;89(2):195-202. doi: 10.1590/s0074-02761994000200013.
Detection of papillomavirus DNA in situ hybridization technique was performed in 29 symptomatic patients (6 males and 23 females) during the period of 1989-1991 at the Clinic for Sexually Transmitted Diseases, Universidade Federal Fluminense, State of Rio de Janeiro. All the male patients had condyloma acuminata. Only HPV 6/11 were found in these lesions. Clinical features in the female patients included vulvar condyloma acuminata, bowenoid papulosis, flat cervical condyloma, cervical condyloma acuminatum and cervical intraepithelial neoplasia grade II (CIN II). We also found cases of condyloma acuminata associated to vulvar intraepithelial neoplasia grade III (VIN III), as well as to vaginal invasive carcinoma. HPV 6/11 and 16/18 were found in vulvar condyloma acuminata. Mixed infection by 6/11-16/18 HPV were also seen in these lesions as well as in the patient who had cervical condyloma acuminatum. HPV 16/18 were found in the condyloma acuminatum plus VIN III and in the CIN II lesions. We have found HPV 31/33/51 in the specimen of condyloma acuminatum plus invasive carcinoma. In order to investigate the ultrastructural aspects of HPV infection in genital tissue, the biopsies of three female patients were observed under electron microscope. Mature virus particles were found in the cells of a condyloma acuminatum as well as in the condyloma acuminatum plus invasive carcinoma case. In another sample, chromosome breakages were found in the nuclei of the infected cells although no viral particles were observed.
1989年至1991年期间，在里约热内卢州弗卢米嫩塞联邦大学性传播疾病诊所，对29例有症状的患者（6名男性和23名女性）进行了乳头瘤病毒DNA原位杂交技术检测。所有男性患者均患有尖锐湿疣。在这些病变中仅发现了HPV 6/11。女性患者的临床特征包括外阴尖锐湿疣、鲍温样丘疹病、扁平宫颈湿疣、宫颈尖锐湿疣和宫颈上皮内瘤变II级（CIN II）。我们还发现了与外阴上皮内瘤变III级（VIN III）以及阴道浸润癌相关的尖锐湿疣病例。在外阴尖锐湿疣中发现了HPV 6/11和16/18。在这些病变以及患有宫颈尖锐湿疣的患者中也观察到了6/11 - 16/18 HPV的混合感染。在尖锐湿疣加VIN III和CIN II病变中发现了HPV 16/18。在尖锐湿疣加浸润癌的标本中发现了HPV 31/33/51。为了研究生殖器组织中HPV感染的超微结构方面，对三名女性患者的活检组织进行了电子显微镜观察。在一例尖锐湿疣以及尖锐湿疣加浸润癌病例的细胞中发现了成熟的病毒颗粒。在另一个样本中，尽管未观察到病毒颗粒，但在受感染细胞的细胞核中发现了染色体断裂。
