Onishi A, Takeda K, Komatsu M, Akita T, Kojima T
Department of Animal Breeding, National Institute of Animal Industry, Ibaraki-ken, Japan.
Biol Reprod. 1994 Dec;51(6):1069-75. doi: 10.1095/biolreprod51.6.1069.
Two injection methods were examined for making chimeras between Chinese pigs (Meishan) and European pigs (Landrace or Landrace x Large White). Furthermore, mitochondrial DNA (mtDNA) polymorphism was detected as a cell marker for the analysis of chimerism. In the first experiment, blastomeres were transplanted into embryos at the 4-16-cell stage. Of 41 transplanted embryos transferred into 3 females, 12 were single-colored, but no overt chimeras were obtained. Judging from coat color and mtDNA in white blood cells, 2 piglets in 2 litters were derived from injected blastomeres, and 10 piglets in 3 litters were derived from recipient blastomeres. In the second experiment, inner cell mass cells of Day 6 Landrace embryos were injected into blastocoels of Day 6 Meishan embryos. Of 35 injected embryos transferred into 3 females, 2 overt chimeras of each sex were obtained in a single litter. In the overt male chimera, mtDNA clearly showed chimerism in spleen, pancreas, brain, kidney, lung, liver, heart, testis, and small intestine. The overt female chimera showed chimerism not only in blood but also in germ line according to a progeny test. No chimerism was detected in any of the 21 single-colored piglets in the second experiment.
研究了两种注射方法，用于培育中国猪（梅山猪）与欧洲猪（长白猪或长白猪×大白猪）之间的嵌合体。此外，检测线粒体DNA（mtDNA）多态性作为分析嵌合现象的细胞标记。在第一个实验中，将卵裂球移植到4-16细胞期的胚胎中。在移植到3头母猪体内的41个移植胚胎中，12个是单色的，但未获得明显的嵌合体。从白细胞的毛色和mtDNA判断，2窝中的2头仔猪来源于注射的卵裂球，3窝中的10头仔猪来源于受体卵裂球。在第二个实验中，将第6天的长白猪胚胎的内细胞团细胞注射到第6天的梅山猪胚胎的囊胚腔中。在移植到3头母猪体内的35个注射胚胎中，一窝中获得了2只每种性别的明显嵌合体。在明显的雄性嵌合体中，mtDNA在脾脏、胰腺、大脑、肾脏、肺、肝脏、心脏、睾丸和小肠中均明显显示出嵌合现象。根据后代检测，明显的雌性嵌合体不仅在血液中而且在生殖系中显示出嵌合现象。在第二个实验的21只单色仔猪中均未检测到嵌合现象。
