Erdtmann M, Keller R, Baumann H
RWTH Aachen, Germany.
Biomaterials. 1994 Oct;15(13):1043-8. doi: 10.1016/0142-9612(94)90089-2.
Heparin (HE), dextran sulphate (DX) of molecular weight 40000 and 500000, dermatan sulphate (DS) and endothelial cell surface heparan sulphate (ES-HS) were immobilized covalently onto cellulose membranes (Visking dialysis tubes) using the photochemical heterobifunctional reagent 4-azido-1-fluoro-2-nitrobenzene (AFNB); 120 pmol HE/cm2 and 40 pmol DS/cm2, 3.4 pmol DX 500,000/cm2, 50 pmol DX 40,000/cm2 and 3.6 pmol ES-HS/cm2 were immobilized. The platelet adhesion of the modified membranes was measured in a modified Baumgartner perfusion chamber with citrated human blood at a defined shear rate. Membranes modified with DX 40,000 and DX 500,000 showed 80% and 30% platelet adhesion, respectively, heparinized and DS coated membranes showed 50% and 60% platelet adhesion, respectively, compared with a subendothelial matrix (100% platelet adhesion). ES-HS modified membranes showed no platelet adhesion.
使用光化学异双功能试剂4-叠氮基-1-氟-2-硝基苯（AFNB）将肝素（HE）、分子量为40000和500000的硫酸葡聚糖（DX）、硫酸皮肤素（DS）和内皮细胞表面硫酸乙酰肝素（ES-HS）共价固定在纤维素膜（维氏透析管）上；固定的量分别为：120 pmol HE/cm²、40 pmol DS/cm²、3.4 pmol DX 500000/cm²、50 pmol DX 40000/cm²和3.6 pmol ES-HS/cm²。在改良的鲍姆加特纳灌注室中，以规定的剪切速率对枸橼酸化人血测量修饰膜的血小板黏附情况。与内皮下基质（血小板黏附率为100%）相比，用DX 40000和DX 500000修饰的膜的血小板黏附率分别为80%和30%，肝素化膜和DS包被膜的血小板黏附率分别为50%和60%。ES-HS修饰的膜未显示血小板黏附。
