通过铬释放抑制试验测定单纯疱疹病毒感染细胞提取物中的病毒特异性抗原。
Determination of virus-specific antigens in extracts from herpes simplex virus-infected cells by a Cr release inhibition test.
作者信息
Kutinová L, Vonka V
出版信息
Infect Immun. 1978 Jun;20(3):587-91. doi: 10.1128/iai.20.3.587-591.1978.
Abstract
A 51cr release inhibition test (CRIT) was developed for determining herpes simplex virus type 1 antigens in infected cell extracts. Among the factors studied were the optimal concentrations of complement, antibody, and infected target cells. Under standardized conditions, the relationship between antigen content and inhibition of chromium release was linear in a semilogarithmic plot, indicating that the antigen content can be determined from testing two dilutions of a given preparation. Other tests showed that infected cells cryopreserved at -70 degrees C for periods exceeding 3 months can be used in CRIT. Five different batches of infected cell extracts were tested in both CRIT and blocking neutralization tests with comparable results being obtained, suggesting that both tests measure the same activity.
摘要
开发了一种51铬释放抑制试验（CRIT），用于测定感染细胞提取物中的1型单纯疱疹病毒抗原。研究的因素包括补体、抗体和感染靶细胞的最佳浓度。在标准化条件下，抗原含量与铬释放抑制之间的关系在半对数图中呈线性，这表明可以通过检测给定制剂的两种稀释度来确定抗原含量。其他试验表明，在-70℃下冷冻保存超过3个月的感染细胞可用于CRIT。对五批不同的感染细胞提取物进行了CRIT和阻断中和试验，结果相当，表明这两种试验测量的是相同的活性。
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