Kutinová L, Slichtová V, Vonka V
Arch Virol. 1979;61(1-2):141-7. doi: 10.1007/BF01320598.
Production of neutralizing antibodies was followed in guinea pigs, rabbits, hamsters and mice immunized with crude antigen extracts (AM) from human diploid cells infected with herpes simplex virus type 1. The AM induced relatively high levels of neutralizing antibodies in all four species. The antibodies were predominantly complement-requiring and remained so even after administration of repeated AM doses. With the strains used, the antibody response was predominantly type specific and, surprisingly, the type specificity of sera usually increased after administration of repeated doses of AM. Guinea pigs seemed to be the best responsive animal species. They developed the highest levels of antibodies and complement-nonrequiring antibodies were seen in them earlier than in the other animal species. The dose-response experiments carried out in guinea pigs indicated that after a single dose administration the ratio between complement-requiring and complement-nonrequiring antibodies was dependent on the amount of antigen administered. When AM was given without adjuvant less efficient antibody production wws observed than after the administration of the same amount of antigen with adjuvant.
在用感染1型单纯疱疹病毒的人二倍体细胞的粗抗原提取物（AM）免疫的豚鼠、兔子、仓鼠和小鼠中，监测了中和抗体的产生情况。AM在所有这四个物种中诱导出相对较高水平的中和抗体。这些抗体主要是补体依赖型的，即使在重复给予AM剂量后仍然如此。对于所使用的毒株，抗体反应主要是型特异性的，令人惊讶的是，血清的型特异性通常在重复给予AM剂量后会增加。豚鼠似乎是反应最佳的动物物种。它们产生的抗体水平最高，并且与其他动物物种相比，在豚鼠中更早出现非补体依赖型抗体。在豚鼠中进行的剂量反应实验表明，单次给药后，补体依赖型抗体与非补体依赖型抗体的比例取决于所给予抗原的量。当无佐剂给予AM时，观察到的抗体产生效率低于给予相同量抗原并添加佐剂的情况。
