Chang K J, Lee T T, Linares-Cruz G, Fournier S, de Ligniéres B
National Taiwan University Hospital, Taipei.
Fertil Steril. 1995 Apr;63(4):785-91.
To study the effect of E2 and P on the epithelial cell cycle of normal human breast in vivo.
Double-blind, randomized study. Topical application to the breast of a gel containing either a placebo, E2, P, or a combination of E2 and P, daily, during the 10 to 13 days preceding breast surgery.
Forty premenopausal women undergoing breast surgery for the removal of a lump. MAIN OUTCOME MEASURES. Plasma and breast tissue concentrations of E2 and P. Epithelial cell cycle evaluated in normal breast tissue areas by counting mitoses and proliferating cell nuclear antigen immunostaining quantitative analyses.
Increased E2 concentration increases the number of cycling epithelial cells. Increased P concentration significantly decreases the number of cycling epithelial cells.
Exposure to P for 10 to 13 days reduces E2-induced proliferation of normal breast epithelial cells in vivo.
研究雌二醇（E2）和孕酮（P）对正常人体乳腺上皮细胞周期的体内作用。
双盲随机研究。在乳房手术前10至13天，每天将含安慰剂、E2、P或E2与P组合的凝胶局部涂抹于乳房。
40名接受乳房肿块切除手术的绝经前女性。主要观察指标：血浆和乳腺组织中E2和P的浓度。通过计数有丝分裂和增殖细胞核抗原免疫染色定量分析评估正常乳腺组织区域的上皮细胞周期。
E2浓度升高会增加处于细胞周期的上皮细胞数量。P浓度升高会显著减少处于细胞周期的上皮细胞数量。
在体内，暴露于P 10至13天可降低E2诱导的正常乳腺上皮细胞增殖。
