突触结合蛋白IV是一种在PC12细胞和大脑中由去极化诱导的即早基因。
Synaptotagmin IV is an immediate early gene induced by depolarization in PC12 cells and in brain.
作者信息
Vician L, Lim I K, Ferguson G, Tocco G, Baudry M, Herschman H R
机构信息
Department of Biological Chemistry, University of California, Los Angeles 90024.
出版信息
Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2164-8. doi: 10.1073/pnas.92.6.2164.
Subtractive library construction and differential screening were used to identify a cDNA for a cell type-specific immediate early gene induced in rat PC12 pheochromocytoma cells. Sequencing identified the protein product of this gene as rat synaptotagmin IV (SytIV). Synaptotagmins are synaptic vesicle proteins thought to play a role in depolarization-induced, calcium-mediated exocytosis and neurotransmitter release. SytIV mRNA accumulation is transiently induced in PC12 cells by potassium depolarization, calcium ionophore, ATP, and forskolin. In contrast, growth factors and phorbol 12-myristate 13-acetate induce little or no SytIV mRNA accumulation. Kainic acid-induced seizures in rats are followed by accumulation of SytIV message in the hippocampus and piriform cortex. The SytIV gene may provide a direct link between depolarization-induced neuronal gene expression and subsequent modulation of synaptic structure and function.
采用消减文库构建和差异筛选技术，以鉴定在大鼠嗜铬细胞瘤PC12细胞中诱导表达的一种细胞类型特异性即时早期基因的cDNA。测序结果表明，该基因的蛋白质产物为大鼠突触结合蛋白IV（SytIV）。突触结合蛋白是突触囊泡蛋白，被认为在去极化诱导的、钙介导的胞吐作用和神经递质释放过程中发挥作用。钾离子去极化、钙离子载体、ATP和福斯高林可在PC12细胞中瞬时诱导SytIV mRNA积累。相比之下，生长因子和佛波酯12-肉豆蔻酸酯13-乙酸酯几乎不诱导或不诱导SytIV mRNA积累。大鼠海人酸诱发癫痫发作后，海马和梨状皮质中会积累SytIV信息。SytIV基因可能在去极化诱导的神经元基因表达与随后突触结构和功能的调节之间提供直接联系。
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