Lauro G M, Solheid C, Medolago-Albani L, Di Lorenzo N, Guidetti B
Acta Neuropathol. 1983;59(2):127-32. doi: 10.1007/BF00691598.
Several human cell lines (normal and neoplastic glia, cerebral metastases from adenocarcinoma, fibroblasts) were incubated with sera from patients with well and poorly differentiated glioma and with sera from healthy donors and then stained with PAP complex to define and localize the antibody reaction with cell surface antigens by means of electron microscopy. The sera of glioma patients proved to contain antibodies which bound the tumor-associated antigenic determinants on the cell membranes of gliomas and of cerebral metastases from adenocarcinoma in tissue cultures. Further, absorption testing of the reactive sera on normal brain, well-differentiated astrocytoma and cultured glioblastoma cells, together with cross-reactivity experiments suggests that at least two antigens or groups of antigens are expressed on the glioma cell surface: one shared by well and poorly differentiated glioma cells and the other by poorly differentiated glioma cells and the cells of cerebral metastases from adenocarcinoma.
将几种人类细胞系（正常和肿瘤性神经胶质细胞、腺癌脑转移瘤细胞、成纤维细胞）与高分化和低分化神经胶质瘤患者的血清以及健康供体的血清一起孵育，然后用PAP复合物染色，通过电子显微镜确定并定位抗体与细胞表面抗原的反应。结果证明，神经胶质瘤患者的血清含有能与组织培养中神经胶质瘤和腺癌脑转移瘤细胞膜上的肿瘤相关抗原决定簇结合的抗体。此外，用正常脑组织、高分化星形细胞瘤和培养的胶质母细胞瘤细胞对反应性血清进行吸收试验以及交叉反应实验表明，神经胶质瘤细胞表面至少表达两种抗原或抗原组：一种为高分化和低分化神经胶质瘤细胞所共有，另一种为低分化神经胶质瘤细胞和腺癌脑转移瘤细胞所共有。
