Dawson J M, Griffiths M J
Regional Cytogenetics Unit, Birmingham Maternity Hospital, Edgbaston, U.K.
Prenat Diagn. 1994 Dec;14(12):1163-5. doi: 10.1002/pd.1970141211.
A simplified method is described for processing both direct preparations and long-term cultures from the same fragment of chorionic villi. Enzyme separation of the outer trophoblast layers (used for direct preparations) from the inner mesenchymal core (used to initiate long-term cultures) facilitates the utilization of the same fragments for the two procedures, without jeopardizing the success of either method. This has proved useful in cases where the sample was so small that only one method of chromosome preparation may have been possible using other techniques.
本文描述了一种简化方法，用于处理来自同一绒毛膜绒毛片段的直接制片和长期培养。通过酶法将外层滋养层（用于直接制片）与内层间充质核心（用于启动长期培养）分离，便于将同一片段用于这两种操作，而不会影响任何一种方法的成功率。在样本非常小以至于使用其他技术可能只能采用一种染色体制片方法的情况下，这种方法已证明是有用的。
