Tsugawa K, Fushida S, Yonemura Y
Second Department of Surgery, School of Medicine, Kanazawa University, Japan.
Oncology. 1993 Nov-Dec;50(6):418-25. doi: 10.1159/000227222.
c-erbB-2 gene amplification was examined by slot blot hybridization using DNAs extracted from formalin-fixed and paraffin-embedded tissues of primary gastric carcinomas. Eleven of the 82 carcinomas (13.4%) exhibited amplification. Twenty-eight carcinomas (34.1%) expressed c-erbB-2 protein immunohistochemically. All of the tumors with amplified c-erbB-2 genes had expression of c-erbB-2 protein. The incidence of diploid and aneuploid tumors with c-erbB-2 gene amplification was similar. The survival rate was significantly lower in cases with c-erbB-2 gene amplification. Furthermore, patients with tumors showing a combination of c-erbB-2 gene amplification and DNA aneuploidy had the poorest prognosis. c-erbB-2 gene amplification may be an important prognostic indicator in gastric carcinomas.
采用从原发性胃癌的福尔马林固定石蜡包埋组织中提取的DNA，通过斑点杂交法检测c-erbB-2基因扩增情况。82例癌组织中有11例（13.4%）出现扩增。28例癌组织（34.1%）免疫组化检测显示c-erbB-2蛋白表达阳性。所有c-erbB-2基因扩增的肿瘤均有c-erbB-2蛋白表达。c-erbB-2基因扩增的二倍体和非整倍体肿瘤的发生率相似。c-erbB-2基因扩增患者的生存率显著较低。此外，肿瘤同时出现c-erbB-2基因扩增和DNA非整倍体的患者预后最差。c-erbB-2基因扩增可能是胃癌的一个重要预后指标。
