Chart H, Conway D, Rowe B
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK.
Epidemiol Infect. 1993 Dec;111(3):449-54. doi: 10.1017/s0950268800057174.
Strains of Salmonella enteritidis belonging to phage type 4 (SE4) were grown in the peritoneal cavities of chickens, and without subculture on laboratory media examined for inducible in vivo phenotypic characteristics. These bacteria expressed three major outer membrane proteins (OMPs) of 33, 35 and 36 kilodaltons (kDa), and iron regulated OMPs of 74, 78 and 81 kDa. Bacteria growing in vivo did not express flagella, or fimbriae with a subunit molecular mass of 14 kDa (14 kDa fimbriae). Two OMPs of 55 and 23 kDa, expressed during culture in nutrient broth, were repressed during growth in chickens. Possession of a 38 MDa 'mouse virulence' plasmid did not influence the expression of OMPs, flagella or fimbriae. It was concluded that strains of SE4 growing in chicken tissues, use an enterobactin mediated iron uptake system to obtain ferric ions, do not express flagella or 14 kDa fimbriae and appear not to express novel OMPs involved in survival in vivo.
属于4型噬菌体的肠炎沙门氏菌菌株在鸡的腹腔中生长，且未经实验室培养基传代培养，而是检测其体内可诱导的表型特征。这些细菌表达三种主要的外膜蛋白（OMP），分子量分别为33、35和36千道尔顿（kDa），以及铁调节的分子量为74、78和81 kDa的OMP。体内生长的细菌不表达鞭毛或亚基分子量为14 kDa的菌毛（14 kDa菌毛）。在营养肉汤中培养时表达的两种分子量为55和23 kDa的OMP，在鸡体内生长过程中受到抑制。拥有一个38 MDa的“小鼠毒力”质粒并不影响OMP、鞭毛或菌毛的表达。得出的结论是，在鸡组织中生长的SE4菌株利用肠杆菌素介导的铁摄取系统获取铁离子，不表达鞭毛或14 kDa菌毛，且似乎不表达参与体内存活的新型OMP。
