Ferea T, Contreras E T, Oung T, Bowman E J, Bowman B J
Department of Biology, Sinsheimer Laboratories, University of California, Santa Cruz 95064.
Mol Gen Genet. 1994 Jan;242(1):105-10. doi: 10.1007/BF00277354.
We have isolated the cDNA and corresponding genomic DNA encoding citrate synthase in Neurospora crassa. Analysis of the protein coding region of this gene, named cit-1, indicates that it specifies the mitochondrial form of citrate synthase. The predicted protein has 469 amino acids and a molecular mass of 52,002 Da. The gene is interrupted by four introns. Hybridization experiments show that a cit-1 probe binds to two different fragments of genomic DNA, which are located on different chromosomes. Neurospora crassa may have two isoforms of citrate synthase, one in the mitochondria and the other in microbodies.
我们已经分离出了粗糙脉孢菌中编码柠檬酸合酶的cDNA及相应的基因组DNA。对这个名为cit-1的基因的蛋白质编码区进行分析表明，它编码的是线粒体形式的柠檬酸合酶。预测的蛋白质有469个氨基酸，分子量为52,002道尔顿。该基因被四个内含子打断。杂交实验表明，cit-1探针与基因组DNA的两个不同片段结合，这两个片段位于不同的染色体上。粗糙脉孢菌可能有两种柠檬酸合酶同工型，一种在线粒体中，另一种在微体中。
