健康志愿者单次剂量与三天泼尼松龙治疗的效果比较：对循环淋巴细胞亚群的不同影响
Effects of single dose compared with three days' prednisolone treatment of healthy volunteers: contrasting effects on circulating lymphocyte subsets.
作者信息
Pountain G D, Keogan M T, Hazleman B L, Brown D L
机构信息
Rheumatology Research Unit, Addenbrooke's Hospital, Cambridge.
出版信息
J Clin Pathol. 1993 Dec;46(12):1089-92. doi: 10.1136/jcp.46.12.1089.
AIMS
To investigate the effects of longer term corticosteroid treatment on circulating lymphocyte subsets.
METHODS
Prednisolone (20 mg daily) was given to 12 healthy volunteers in a single morning dose for three days. Circulating lymphocyte subsets were measured by flow cytometry after whole blood lysis.
RESULTS
Seven hours after the first dose of prednisolone there was a significant fall in absolute numbers of lymphocytes, T cells, CD4+ and CD8+ cells, and B cells. The percentage of T cells fell significantly, due to a fall in percentage of CD4+ cells. In contrast to the seven hour findings, at 72 hours there was a significant rise in absolute numbers of lymphocytes, T cells, CD4+, CD8+, and B cells. This trend was already apparent by 24 hours. The percentage of CD4+ cells was significantly raised at 72 hours, while that of CD8+ cells had fallen significantly. The percentage of natural killer cells had fallen at 72 hours; that of B cells remained increased at 72 hours.
CONCLUSIONS
These findings show that corticosteroid treatment causes significant changes in lymphocyte subsets, and that such changes must be considered when designing studies of lymphocyte subsets during illness.
目的
研究长期使用皮质类固醇治疗对循环淋巴细胞亚群的影响。
方法
12名健康志愿者每日单次晨起服用泼尼松龙（20毫克），共服用三天。全血裂解后通过流式细胞术检测循环淋巴细胞亚群。
结果
首次服用泼尼松龙7小时后，淋巴细胞、T细胞、CD4⁺和CD8⁺细胞以及B细胞的绝对数量显著下降。由于CD4⁺细胞百分比下降，T细胞百分比显著下降。与7小时的结果相反，在72小时时，淋巴细胞、T细胞、CD4⁺、CD8⁺和B细胞的绝对数量显著增加。这一趋势在24小时时已经明显。72小时时CD4⁺细胞百分比显著升高，而CD8⁺细胞百分比显著下降。72小时时自然杀伤细胞百分比下降；B细胞百分比在72小时时仍升高。
结论
这些发现表明，皮质类固醇治疗会导致淋巴细胞亚群发生显著变化，并且在设计疾病期间淋巴细胞亚群的研究时必须考虑到这些变化。
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