Garg R P, Menon A L, Jacobs K, Robson R M, Robson R L
Department of Biochemistry, University of Georgia, Athens 30602-7229.
J Mol Biol. 1994 Feb 11;236(1):390-6. doi: 10.1006/jmbi.1994.1149.
The nucleotide sequence was obtained for the hypE gene in the cluster of structural and accessory genes required for the assembly and functioning of the membrane-bound, dimeric, (NiFe)hydrogenase in Azotobacter vinelandii. The hypE gene encodes a polypeptide of 341 amino acid residues which is rich in alanine, glycine, valine and proline and appears to be involved in maturation of the enzyme because chromosomal mutations in hypE block O2-dependent H2-oxidation and affect the amount, processing and localization of the (NiFe) hydrogenase alpha-subunit. The complete nucleotide sequence for the hydrogenase gene cluster in A. vinelandii has now been assembled into a contiguous sequence of 13,914 bp containing 16 potential genes which appear to be transcribed undirectionally. They are arranged in the order hoxK, hoxG, hoxZ, hoxM, hoxL, hoxO, hoxQ, hoxR, hoxT, hoxV, hypA, hypB, hypF, hypC, hypD and hypE. This cluster closely resembles those described for comparable (NiFe) hydrogenases in other bacteria.
获得了棕色固氮菌中膜结合二聚体（NiFe）氢化酶组装和功能所需的结构及辅助基因簇中hypE基因的核苷酸序列。hypE基因编码一个由341个氨基酸残基组成的多肽，该多肽富含丙氨酸、甘氨酸、缬氨酸和脯氨酸，似乎参与了该酶的成熟过程，因为hypE中的染色体突变会阻断依赖氧气的H2氧化，并影响（NiFe）氢化酶α亚基的数量、加工和定位。棕色固氮菌中氢化酶基因簇的完整核苷酸序列现已组装成一个13914 bp的连续序列，其中包含16个潜在基因，这些基因似乎是单向转录的。它们按hoxK、hoxG、hoxZ、hoxM、hoxL、hoxO、hoxQ、hoxR、hoxT、hoxV、hypA、hypB、hypF、hypC、hypD和hypE的顺序排列。该基因簇与其他细菌中类似的（NiFe）氢化酶所描述的基因簇非常相似。
