编码大肠杆菌氢化酶2的hyb操纵子的克隆、测序及突变分析。
Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2.
作者信息
Menon N K, Chatelus C Y, Dervartanian M, Wendt J C, Shanmugam K T, Peck H D, Przybyla A E
机构信息
Department of Biochemistry, University of Georgia, Athens 30602.
出版信息
J Bacteriol. 1994 Jul;176(14):4416-23. doi: 10.1128/jb.176.14.4416-4423.1994.
The genes encoding the two structural subunits of Escherichia coli hydrogenase 2 (HYD2) have been cloned and sequenced. They occur in an operon (hyb) which contains seven open reading frames. An hyb deletion mutant (strain AP3) failed to grown on dihydrogen-fumarate medium and also produced very low levels of HYD1. All seven open reading frames are required for restoration of wild-type levels of active HYD2 in AP3. The hyb operon was mapped at 65 min on the E. coli chromosome.
编码大肠杆菌氢化酶2（HYD2）两个结构亚基的基因已被克隆和测序。它们存在于一个包含七个开放阅读框的操纵子（hyb）中。一个hyb缺失突变体（菌株AP3）无法在富马酸二氢培养基上生长，并且产生的HYD1水平也非常低。AP3中活性HYD2的野生型水平恢复需要所有七个开放阅读框。hyb操纵子定位于大肠杆菌染色体的65分钟处。
Zotero 插件