鉴定针对γ/δT细胞受体的单克隆抗体。
Identification of monoclonal antibodies specific for the gamma/delta TCR.
作者信息
Parsons K R, Crocker G, Sopp P, Howard C J, Davis W C
机构信息
AFRC Institute for Animal Health, Compton, Nr Newbury, UK.
出版信息
Vet Immunol Immunopathol. 1993 Nov;39(1-3):161-7. doi: 10.1016/0165-2427(93)90177-6.
Twelve monoclonal antibodies (mAbs) formed temporary cluster TC36. mAb CACTB6A was included as a standard antibody that reacted with the gamma/delta T cell receptor (TCR). Eight of the mAbs, CACTB6A, CACT16A, CACT17A, CACTB12A, CACTB44A, CACT71A, CACT18A and CACT61A, immunoprecipitated a 47 kDa molecule indicating that they all recognize the gamma/delta TCR. Flow cytometric studies separated these mAbs into three groups that distinguish three different gamma/delta TCR. mAb BAQ72A, which fell within TC36, immunoprecipitated a 215 kDa molecule with a similar tissue and cellular distribution to BoWC1 mAb. mAbs CACT26A, CACT63A and CACT77A reacted similarly to each other by flow cytometry and immunohistology, but were distinct from the other mAbs in TC36. These three mAbs may recognize an activation molecule and form a distinct novel mAb cluster.
12种单克隆抗体（mAb）形成了临时簇TC36。单克隆抗体CACTB6A作为与γ/δT细胞受体（TCR）反应的标准抗体被纳入。其中8种单克隆抗体，即CACTB6A、CACT16A、CACT17A、CACTB12A、CACTB44A、CACT71A、CACT18A和CACT61A，免疫沉淀出一个47 kDa的分子，表明它们都识别γ/δTCR。流式细胞术研究将这些单克隆抗体分为三组，区分出三种不同的γ/δTCR。属于TC36的单克隆抗体BAQ72A免疫沉淀出一个215 kDa的分子，其组织和细胞分布与BoWC1单克隆抗体相似。单克隆抗体CACT26A、CACT63A和CACT77A在流式细胞术和免疫组织化学方面彼此反应相似，但与TC36中的其他单克隆抗体不同。这三种单克隆抗体可能识别一种激活分子并形成一个独特的新型单克隆抗体簇。
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