小鼠近端6号染色体的显微切割：与ob突变紧密连锁的限制性片段长度多态性的鉴定

Microdissection of proximal mouse chromosome 6: identification of RFLPs tightly linked to the ob mutation.

作者信息

Bahary N, Siegel D A, Walsh J, Zhang Y, Leopold L, Leibel R, Proenca R, Friedman J M

机构信息

Laboratory of Molecular Genetics, Rockefeller University, New York, New York 10021.

出版信息

Mamm Genome. 1993 Sep;4(9):511-5. doi: 10.1007/BF00364786.

DOI:10.1007/BF00364786

PMID:7906968

Abstract

In a previous report, the ob mutation was mapped to a position 5 cM distal to Met on murine Chromosome (Chr) 6 in tight linkage to Cpa. In order to identify additional RFLPs in the region of ob, we have made use of chromosome microdissection of a 6:16 Robertsonian chromosome. In total, 19 RFLPs were used to type 131 progency of a B6D2 ob/ + x B6 spretus ob/ + intercross. Fifteen of the RFLPs mapped to Chr 6, one of which, D6Rck13, was tightly linked to ob. For refinement of the genetic map around ob, 350 obese progency of a B6 Mus castaneus ob/ + intercross were characterized. DNAs from these animals were typed for microsatellite markers from Chr 6 that flank ob. Recombinants were then typed for D6Rck13. D6Rck13 was nonrecombinant among all the progency of both crosses corresponding to 831 meioses. This probe will be of use as an entry point for physical mapping of the ob mutation.

摘要

在之前的一份报告中，ob 突变被定位到小鼠 6 号染色体（Chr）上距 Met 远端 5 cM 的位置，与 Cpa 紧密连锁。为了在 ob 区域鉴定更多的限制性片段长度多态性（RFLP），我们利用了一条 6:16 罗伯逊易位染色体进行染色体显微切割。总共使用了 19 个 RFLP 对 B6D2 ob/+×B6 spretus ob/+杂交的 131 个后代进行分型。其中 15 个 RFLP 定位到 6 号染色体，其中一个，即 D6Rck13，与 ob 紧密连锁。为了完善 ob 周围的遗传图谱，对 B6 栗色小鼠 ob/+杂交的 350 个肥胖后代进行了特征分析。对这些动物的 DNA 进行了 6 号染色体上位于 ob 两侧的微卫星标记分型。然后对重组体进行 D6Rck13 分型。在对应于 831 次减数分裂的两个杂交的所有后代中，D6Rck13 均无重组。该探针将作为 ob 突变物理图谱绘制的切入点。