Liu C Y, Grant A L, Kim K H, Mills S E
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907.
Domest Anim Endocrinol. 1994 Jan;11(1):125-32. doi: 10.1016/0739-7240(94)90040-x.
Crossbred barrows were treated daily with porcine somatotropin (pST; 4 mg/d) from 79 to 127 kg BW to determine whether pST regulates the activity and gene expression of adipose tissue acetyl-CoA carboxylase (ACC), the rate limiting enzyme in de novo fatty acid synthesis. Administration of pST reduced ACC enzyme activity, protein content, and mRNA abundance in adipose tissue by 40 to 50%. When comparisons were made among all pigs, ACC enzyme activity and mRNA abundance were closely associated (r2 = .94). In summary, our results indicate that pST decreases ACC enzyme activity and that this is associated with a significant reduction in ACC mRNA abundance. We speculate that decreased ACC enzyme activity results from a reduction in ACC protein and that this occurs because pST reduces the abundance of mRNA available for translation.
将体重79至127千克的杂交公猪每天用猪生长激素（pST；4毫克/天）进行处理，以确定pST是否调节脂肪组织乙酰辅酶A羧化酶（ACC，从头脂肪酸合成中的限速酶）的活性和基因表达。施用pST可使脂肪组织中的ACC酶活性、蛋白质含量和mRNA丰度降低40%至50%。在所有猪之间进行比较时，ACC酶活性和mRNA丰度密切相关（r2 = 0.94）。总之，我们的结果表明，pST降低了ACC酶活性，这与ACC mRNA丰度的显著降低有关。我们推测，ACC酶活性降低是由于ACC蛋白质减少所致，而这是因为pST降低了可供翻译的mRNA丰度。
