Nakasone Noboru, Iwanaga Masaaki, Yamashiro Tetsu, Nakashima Kazutoshi, Albert M John
Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Okinawa 903-01, Japan.
Research Center of Comprehensive Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa 903-01, Japan.
Microbiology (Reading). 1996 Feb;142 ( Pt 2):309-313. doi: 10.1099/13500872-142-2-309.
Pili of Aeromonas trota strain 1220, which agglutinates with Vibrio cholerae O139 Bengal antiserum, were purified and characterized. The molecular mass of the subunit protein was estimated to be 20 kDa and the pl was 5 center dot 4. The pili were immunologically unrelated to the other Aeromonas pili reported so far. However, the N-terminal amino acid sequence of the subunit pilin was similar to those of the pilins from other Aeromonas pili reported previously. Neither A. trota cells nor pili purified from strain 1220 agglutinated human and rabbit erythrocytes, but both adhered to the rabbit intestine. Bacterial cells pretreated with antipilus antibody (Fab portion) failed to adhere to the rabbit intestine. Moreover, bacteria did not adhere to the rabbit intestine pretreated with the purified pili. This pilus antigen was not detected in V. cholerae O139 Bengal and other Aeromonas spp. These findings suggest that the pilus of the A. trota strain is a novel colonization factor of Aeromonas spp.
与霍乱弧菌O139孟加拉型抗血清发生凝集反应的豚鼠气单胞菌1220株菌毛被纯化并进行了特性分析。亚基蛋白的分子量估计为20 kDa，等电点为5.4。这些菌毛在免疫上与迄今报道的其他气单胞菌菌毛无关。然而，亚基菌毛蛋白的N端氨基酸序列与先前报道的其他气单胞菌菌毛的菌毛蛋白相似。豚鼠气单胞菌细胞和从1220株纯化的菌毛均不凝集人和兔的红细胞，但二者均能黏附于兔肠道。用抗菌毛抗体（Fab段）预处理的细菌细胞不能黏附于兔肠道。此外，细菌不能黏附于用纯化菌毛预处理的兔肠道。在霍乱弧菌O139孟加拉型和其他气单胞菌属中未检测到这种菌毛抗原。这些发现表明豚鼠气单胞菌菌株的菌毛是气单胞菌属一种新的定植因子。
