On the metabolic characteristic of hybrids Shigella flexneri x Escherichia coli devoid of their ability for intracellular multiplication in the epithelial cells. I. Glycolysis.

The glycolysis (aerobic and in the presence of KCN) of avirulent xyl+ and xyl-str hybrids Sh. flexneri x E. coli, devoid of their ability to multiply intracellularly in the epithelial cells, is studied. It is established that their glycolytic activity is considerably higher than the activity of the donor strain E. coli, and deviate significantly compared with shown activity of the virulent recipient strains Sh. flexneri. Xyl-str hybrids are showing high values of glycolysis in aerobic conditions and in the presence of KCN, while in xyl+ hybrids very active glycolysis is observed only in the presence of KCN - probably completely sufficient for the persistence of bacteria in the host-cell at anaerobic conditions.