Dong Z W, Li Y, Zhang L Y, Liu R M
Department of Molecular Biology, National Research Institute for Family Planning, Beijing, China.
Int J Gynaecol Obstet. 1998 Apr;61(1):29-32. doi: 10.1016/s0020-7292(98)00019-8.
To detect Chlamydia trachomatis intrauterine infection in the early pregnancy by using chorionic villi.
The C. trachomatis infection in pregnant women was investigated by cervical specimens and Clearview kits. DNA of chorionic villi was extracted and the gene of a major outer membrane protein of C. trachomatis was amplified by polymerase chain reaction (PCR).
120 cervical specimens of the pregnant women were analyzed and 10 cervical specimens were positive for C. trachomatis infection. In this study, the prevalence of C. trachomatis infection was approx. 8.3%. Fifty-nine specimens of chorionic villi and three positive specimens of C. trachomatis infection were analyzed by PCR. The incidence of C. trachomatis intrauterine infection in the early pregnancy was 5.1%.
The vertical transmission of C. trachomatis infection in the early pregnancy may be a pathway of intrauterine infection. Chorionic villus sampling in early pregnancy and the PCR method could be developed as a technique for prenatal diagnosis of C. trachomatis intrauterine infection.
利用绒毛膜检测早期妊娠时沙眼衣原体宫内感染情况。
采用宫颈标本及Clearview试剂盒对孕妇沙眼衣原体感染情况进行调查。提取绒毛膜DNA，应用聚合酶链反应（PCR）扩增沙眼衣原体主要外膜蛋白基因。
对120例孕妇宫颈标本进行分析，10例宫颈标本沙眼衣原体感染呈阳性。本研究中，沙眼衣原体感染率约为8.3%。对59例绒毛膜标本及3例沙眼衣原体感染阳性标本进行PCR分析。早期妊娠时沙眼衣原体宫内感染发生率为5.1%。
早期妊娠时沙眼衣原体感染的垂直传播可能是宫内感染的一条途径。早期妊娠绒毛取样及PCR方法可作为产前诊断沙眼衣原体宫内感染的一项技术来开发。
