Léonard G, Chaput A, Courgnaud V, Sangaré A, Denis F, Brechot C
Laboratoire de Virologie, CHU Dupuytren, Limoges, Paris, France.
AIDS. 1993 Sep;7(9):1185-9. doi: 10.1097/00002030-199309000-00006.
To evaluate the frequency of dual HIV-1 and HIV-2 DNA sequences in patients with dual serological profiles.
We tested 40 samples from AIDS patients living in Abidjan, Côte d'Ivoire.
Dual serological reactivity was determined by double Western blot and two enzyme-linked immunosorbent assays with recombinant proteins and synthetic peptides as antigens. The Western blot was considered to show dual reactivity when sera reacted with at least two glycoproteins and one core protein of each virus. HIV DNA sequences were detected by hybridization to radiolabelled probes of polymerase chain reaction (PCR) products amplified using specific primers.
Both HIV-1 and HIV-2 DNA sequences were detected in four out of 11 samples with a dual serological profile and in four out of 24 samples with anti-HIV-1 antibodies only.
These results show that dual HIV-1 and HIV-2 serological profiles are not always due to infection by both viruses, and emphasize the need for a combination of serological and PCR assays for the appraisal of these viral infections.
评估具有双重血清学特征的患者中HIV-1和HIV-2双重DNA序列的频率。
我们检测了来自科特迪瓦阿比让的40例艾滋病患者的样本。
通过双免疫印迹法以及两种以重组蛋白和合成肽为抗原的酶联免疫吸附测定法来确定双重血清学反应性。当血清与每种病毒的至少两种糖蛋白和一种核心蛋白发生反应时，免疫印迹法被认为显示出双重反应性。通过与使用特异性引物扩增的聚合酶链反应（PCR）产物的放射性标记探针杂交来检测HIV DNA序列。
在11例具有双重血清学特征的样本中有4例同时检测到HIV-1和HIV-2 DNA序列，在仅具有抗HIV-1抗体的24例样本中有4例检测到。
这些结果表明，HIV-1和HIV-2双重血清学特征并非总是由两种病毒感染所致，并强调了需要结合血清学和PCR检测来评估这些病毒感染。
