Moffatt M F, Hill M R, Cornélis F, Schou C, Faux J A, Young R P, James A L, Ryan G, le Souef P, Musk A W
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Headington, Oxford, UK.
Lancet. 1994 Jun 25;343(8913):1597-600. doi: 10.1016/s0140-6736(94)93057-0.
IgE responses to inhaled proteins underlie the clinical syndrome of allergic (atopic) asthma and rhinitis. We have investigated genetic linkage between specific IgE reactions to highly purified major allergens and the T-cell receptor (TCR) alpha and beta gene complexes on chromosome 14 and 7, respectively. Antigens tested included highly purified proteins from the housedust mite Dermatophagoides pteronyssinus, the domestic cat and dog, grass pollen, and the mould Alternaria alternata. Affected sibling-pair methods were used in two independent sets of families, one in the UK and one in Australia. No linkage of IgE serotypes to TCR-beta was detected, but significant linkage to TCR-alpha was seen in both family groups. For several of the IgE phenotypes investigated (positive responses to whole allergen sources or purified antigens or serum IgE above the 70th percentile in the population) the affected sibling-pairs showed significant sharing of TCR-alpha microsatellite alleles from both parents. The results show that a gene (or genes) in the TCR-alpha region modifies specific IgE responses.
对吸入性蛋白质的IgE反应是过敏性（特应性）哮喘和鼻炎临床综合征的基础。我们分别研究了对高度纯化的主要过敏原的特异性IgE反应与位于14号和7号染色体上的T细胞受体（TCR）α和β基因复合体之间的遗传连锁关系。所测试的抗原包括来自屋尘螨、家猫和家犬、草花粉以及链格孢霉菌的高度纯化蛋白质。在两组独立的家庭中采用了受累同胞对法，一组在英国，另一组在澳大利亚。未检测到IgE血清型与TCR-β的连锁关系，但在两个家庭组中均观察到与TCR-α有显著连锁。对于所研究的几种IgE表型（对全过敏原来源或纯化抗原呈阳性反应或血清IgE在人群中高于第70百分位数），受累同胞对显示出从父母双方共享TCR-α微卫星等位基因。结果表明，TCR-α区域中的一个基因（或多个基因）修饰特异性IgE反应。
