Chandler D S, Mynott T L, Luke R K, Craven J A
Victorian Institute of Animal Science, Department of Agriculture, Australia.
Vet Microbiol. 1994 Jan;38(3):203-15. doi: 10.1016/0378-1135(94)90002-7.
The levels of Escherichia coli K88 receptor were measured at various sites within the pig intestinal tract using an enzyme immunoassay. The amount of receptor in samples taken from K88-adhesive phenotype animals was found to vary widely along the length of the intestinal tract, but was usually highest in mucosal scrapings taken from the mid-small intestine. Receptor was evident in material collected near either end of the small intestine and was not apparent in material collected from the caecum or lower bowel. The ability of receptor-containing intestinal material to react with immobilized K88 adhesin was inhibited by exposure of the material to either trypsin or contents from the lower bowel, if the receptor-containing material was reacted with the immobilised K88 adhesin prior to exposure to trypsin or lower bowel contents, the bound material remained evident for 24 to 48 h. The possible implications of variable receptor activity in proteolytic environments in relation to pathogenesis and the determination of K88 phenotype in live pigs is discussed.
采用酶免疫测定法检测猪肠道内不同部位的大肠杆菌K88受体水平。结果发现，从具有K88黏附表型的动物采集的样本中，受体数量沿肠道长度变化很大，但通常在中小肠中部的黏膜刮片中含量最高。在小肠两端附近采集的材料中可检测到受体，而在盲肠或下肠道采集的材料中则未检测到。如果在将含受体的材料暴露于胰蛋白酶或下肠道内容物之前，使其与固定化的K88黏附素反应，那么含受体的肠道材料与固定化K88黏附素反应的能力会受到胰蛋白酶或下肠道内容物的抑制，结合的材料在24至48小时内仍可检测到。本文讨论了在蛋白水解环境中受体活性变化对发病机制以及活猪K88表型测定的可能影响。
