Dean M, Allikmets R, Gerrard B, Stewart C, Kistler A, Shafer B, Michaelis S, Strathern J
Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201.
Yeast. 1994 Mar;10(3):377-83. doi: 10.1002/yea.320100310.
ATP-binding cassette (ABC) transporters share significant sequence identity within their ATP-binding domains. Degenerate oligonucleotides based on highly conserved portions of the ATP-binding domain genes were used to clone portions of two members of the ABC gene superfamily from Saccharomyces cerevisiae DNA. These genes were designated MDL1 and MDL2 (for multidrug resistance-like). Each MDL gene is predicted to encode a single set of transmembrane domains and a single ATP-binding domain, thus the MDL gene products are 'half-molecule' ABC proteins. The two genes were mapped to precise regions on chromosomes XII and XVI and show a considerable similarity to the mammalian P-glycoprotein/multidrug resistance (MDR) and peptide transporter (TAP) genes. Preliminary analysis of null mutants constructed by gene replacement has indicated that the MDL genes are not essential for viability of yeast. The sequences have been deposited in the GenBank data library under Accession Numbers L16958 (Locus YSCBCSA) and L16959 (Locus YSCBCSB).
ATP结合盒（ABC）转运蛋白在其ATP结合结构域内具有显著的序列同一性。基于ATP结合结构域基因高度保守部分的简并寡核苷酸被用于从酿酒酵母DNA中克隆ABC基因超家族两个成员的部分序列。这些基因被命名为MDL1和MDL2（类多药耐药）。每个MDL基因预计编码一组单一的跨膜结构域和一个单一的ATP结合结构域，因此MDL基因产物是“半分子”ABC蛋白。这两个基因被定位到染色体XII和XVI上的精确区域，并且与哺乳动物的P-糖蛋白/多药耐药（MDR）和肽转运体（TAP）基因显示出相当大的相似性。通过基因替换构建的缺失突变体的初步分析表明，MDL基因对于酵母的生存力不是必需的。这些序列已存入GenBank数据库，登录号分别为L16958（基因座YSCBCSA）和L16959（基因座YSCBCSB）。
