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大肠杆菌SecB在枯草芽孢杆菌中的表达促进了大肠杆菌中SecB依赖性麦芽糖结合蛋白的分泌。

Expression of Escherichia coli SecB in Bacillus subtilis facilitates secretion of the SecB-dependent maltose-binding protein of E. coli.

作者信息

Collier D N

机构信息

Central Research and Development Division, E. I. DuPont de Nemours & Co., Wilmington, Delaware 19880-0328.

出版信息

J Bacteriol. 1994 Aug;176(16):4937-40. doi: 10.1128/jb.176.16.4937-4940.1994.

DOI:10.1128/jb.176.16.4937-4940.1994
PMID:7914188
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC196330/
Abstract

Less than 20% of the Escherichia coli maltose-binding protein (MBP) synthesized in Bacillus subtilis is exported. However, a portion of the secreted MBP was processed cotranslationally. Coexpression of SecB, a secretion-related chaperone of E. coli, stimulated posttranslational export of MBP in B. subtilis but inhibited its cotranslational processing. Export of a SecB-independent MBP-ribose-binding protein hybrid precursor was not enhanced by SecB. A slowly folding MBP derivative (MBP-Y283D) was more efficiently secreted than wild-type MBP, suggesting that the antifolding activity of SecB promotes posttranslational secretion of MBP in B. subtilis.

摘要

在枯草芽孢杆菌中合成的大肠杆菌麦芽糖结合蛋白（MBP）只有不到20%被分泌。然而，一部分分泌的MBP是共翻译加工的。大肠杆菌分泌相关伴侣蛋白SecB的共表达刺激了枯草芽孢杆菌中MBP的翻译后分泌，但抑制了其共翻译加工。SecB对不依赖SecB的MBP-核糖结合蛋白杂交前体的分泌没有增强作用。一种折叠缓慢的MBP衍生物（MBP-Y283D）比野生型MBP分泌效率更高，这表明SecB的抗折叠活性促进了枯草芽孢杆菌中MBP的翻译后分泌。

相似文献

1
Expression of Escherichia coli SecB in Bacillus subtilis facilitates secretion of the SecB-dependent maltose-binding protein of E. coli.大肠杆菌SecB在枯草芽孢杆菌中的表达促进了大肠杆菌中SecB依赖性麦芽糖结合蛋白的分泌。
J Bacteriol. 1994 Aug;176(16):4937-40. doi: 10.1128/jb.176.16.4937-4940.1994.
2
SecB-independent export of Escherichia coli ribose-binding protein (RBP): some comparisons with export of maltose-binding protein (MBP) and studies with RBP-MBP hybrid proteins.大肠杆菌核糖结合蛋白（RBP）不依赖SecB的输出：与麦芽糖结合蛋白（MBP）输出的一些比较以及对RBP-MBP杂合蛋白的研究。
J Bacteriol. 1990 Dec;172(12):6875-84. doi: 10.1128/jb.172.12.6875-6884.1990.
3
The antifolding activity of SecB promotes the export of the E. coli maltose-binding protein.SecB的抗折叠活性促进了大肠杆菌麦芽糖结合蛋白的输出。
Cell. 1988 Apr 22;53(2):273-83. doi: 10.1016/0092-8674(88)90389-3.
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Mutations that improve export of maltose-binding protein in SecB- cells of Escherichia coli.改善麦芽糖结合蛋白在大肠杆菌SecB缺陷型细胞中输出的突变。
J Bacteriol. 1989 Sep;171(9):4640-7. doi: 10.1128/jb.171.9.4640-4647.1989.
5
The mature portion of Escherichia coli maltose-binding protein (MBP) determines the dependence of MBP on SecB for export.大肠杆菌麦芽糖结合蛋白（MBP）的成熟部分决定了MBP对SecB的输出依赖性。
J Bacteriol. 1989 Feb;171(2):813-8. doi: 10.1128/jb.171.2.813-818.1989.
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Purified secB protein of Escherichia coli retards folding and promotes membrane translocation of the maltose-binding protein in vitro.大肠杆菌纯化的SecB蛋白在体外可延缓麦芽糖结合蛋白的折叠并促进其膜易位。
Proc Natl Acad Sci U S A. 1988 Dec;85(23):8978-82. doi: 10.1073/pnas.85.23.8978.
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The folding properties of the Escherichia coli maltose-binding protein influence its interaction with SecB in vitro.大肠杆菌麦芽糖结合蛋白的折叠特性在体外影响其与SecB的相互作用。
J Bacteriol. 1990 Jun;172(6):3023-9. doi: 10.1128/jb.172.6.3023-3029.1990.
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Regions of maltose-binding protein that influence SecB-dependent and SecA-dependent export in Escherichia coli.麦芽糖结合蛋白中影响大肠杆菌中SecB依赖性和SecA依赖性输出的区域。
J Bacteriol. 1993 Nov;175(21):6988-95. doi: 10.1128/jb.175.21.6988-6995.1993.
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Mutations of the molecular chaperone protein SecB which alter the interaction between SecB and maltose-binding protein.分子伴侣蛋白SecB的突变会改变SecB与麦芽糖结合蛋白之间的相互作用。
J Biol Chem. 1993 Jan 25;268(3):1590-5.
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Escherichia coli SecB protein associates with exported protein precursors in vivo.大肠杆菌SecB蛋白在体内与输出的蛋白质前体相结合。
Proc Natl Acad Sci U S A. 1989 Jul;86(14):5320-4. doi: 10.1073/pnas.86.14.5320.

引用本文的文献

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Appl Environ Microbiol. 2012 Feb;78(3):651-9. doi: 10.1128/AEM.07209-11. Epub 2011 Nov 23.
2
New integrative method to generate Bacillus subtilis recombinant strains free of selection markers.用于构建无选择标记枯草芽孢杆菌重组菌株的新型整合方法。
Appl Environ Microbiol. 2004 Dec;70(12):7241-50. doi: 10.1128/AEM.70.12.7241-7250.2004.

本文引用的文献

1
Folding of maltose-binding protein. Evidence for the identity of the rate-determining step in vivo and in vitro.麦芽糖结合蛋白的折叠。体内和体外速率决定步骤一致性的证据。
J Biol Chem. 1993 Oct 5;268(28):20855-62.
2
Escherichia coli signal peptides direct inefficient secretion of an outer membrane protein (OmpA) and periplasmic proteins (maltose-binding protein, ribose-binding protein, and alkaline phosphatase) in Bacillus subtilis.大肠杆菌信号肽在枯草芽孢杆菌中导致外膜蛋白（OmpA）和周质蛋白（麦芽糖结合蛋白、核糖结合蛋白和碱性磷酸酶）的分泌效率低下。
J Bacteriol. 1994 May;176(10):3013-20. doi: 10.1128/jb.176.10.3013-3020.1994.
3
SecB: a molecular chaperone of Escherichia coli protein secretion pathway.
SecB：大肠杆菌蛋白质分泌途径的分子伴侣。
Adv Protein Chem. 1993;44:151-93. doi: 10.1016/s0065-3233(08)60567-7.
4
Isolation and characterization of the secE homologue gene of Bacillus subtilis.枯草芽孢杆菌secE同源基因的分离与鉴定
Mol Microbiol. 1993 Oct;10(1):133-42. doi: 10.1111/j.1365-2958.1993.tb00910.x.
5
Processing in vivo of precursor maltose-binding protein in Escherichia coli occurs post-translationally as well as co-translationally.大肠杆菌中前体麦芽糖结合蛋白的体内加工过程在翻译后以及共翻译时都会发生。
J Biol Chem. 1981 Mar 10;256(5):2504-7.
6
Mutations in a new gene, secB, cause defective protein localization in Escherichia coli.一个新基因secB中的突变会导致大肠杆菌中蛋白质定位缺陷。
J Bacteriol. 1983 Apr;154(1):253-60. doi: 10.1128/jb.154.1.253-260.1983.
7
Analysis of cotranslational proteolytic processing of nascent chains using two-dimensional gel electrophoresis.使用二维凝胶电泳分析新生肽链的共翻译蛋白水解加工过程。
Methods Enzymol. 1983;97:77-85. doi: 10.1016/0076-6879(83)97121-5.
8
Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.超螺旋测序：一种用于质粒DNA测序的快速简便方法。
DNA. 1985 Apr;4(2):165-70. doi: 10.1089/dna.1985.4.165.
9
Evidence for specificity at an early step in protein export in Escherichia coli.大肠杆菌中蛋白质输出早期步骤特异性的证据。
J Bacteriol. 1985 Jul;163(1):267-74. doi: 10.1128/jb.163.1.267-274.1985.
10
Suppression of a signal sequence mutation by an amino acid substitution in the mature portion of the maltose-binding protein.麦芽糖结合蛋白成熟部分的氨基酸取代对信号序列突变的抑制作用。
J Bacteriol. 1987 May;169(5):1794-800. doi: 10.1128/jb.169.5.1794-1800.1987.