Monga M, Roberts J A
Department of Urology, Tulane University School of Medicine, New Orleans, Louisiana.
J Androl. 1994 Mar-Apr;15(2):151-6.
The influence of genital infection on infertility has yet to be elucidated. We examined receptor-ligand interactions between sperm and Escherichia coli from patients with prostatitis. Two E. coli surface adhesins (P-fimbriae, type 1 fimbriae) and their specific receptor saccharides (alpha-galp-1-4-beta-galp-O-methyl [gal-gal], mannose) were evaluated. Bacterial concentrations of 10(4) caused spermagglutination. P-fimbriae caused tail-tail spermagglutination that was inhibited by gal-gal. D-mannose concentrations are highest in the acrosomal region and type 1 fimbriae caused head-head agglutination that was inhibited by mannose. Strains with both fimbriae caused head-head and tail-tail agglutination that was inhibited by a mannose/gal-gal combination. E. coli agglutinated 40-75% of motile sperm. Seminal fluid provided 50-100% protection, with lower effectiveness against type 1 fimbriae. Understanding bacteria-spermatozoa interactions at the receptor-ligand level holds potential for treatment of infertility and development of spermagglutinating contraceptives.
生殖系统感染对不孕症的影响尚待阐明。我们研究了前列腺炎患者精子与大肠杆菌之间的受体 - 配体相互作用。评估了两种大肠杆菌表面粘附素（P菌毛、1型菌毛）及其特异性受体糖类（α - 半乳糖 - 1 - 4 - β - 半乳糖 - O - 甲基[半乳糖 - 半乳糖]、甘露糖）。细菌浓度为10⁴时可引起精子凝集。P菌毛引起精子尾 - 尾凝集，可被半乳糖 - 半乳糖抑制。顶体区域的D - 甘露糖浓度最高，1型菌毛引起精子头 - 头凝集，可被甘露糖抑制。同时具有两种菌毛的菌株引起精子头 - 头和尾 - 尾凝集，可被甘露糖/半乳糖 - 半乳糖组合抑制。大肠杆菌凝集40 - 75%的活动精子。精液提供50 - 100%的保护作用，对1型菌毛的保护效果较低。在受体 - 配体水平上了解细菌与精子的相互作用对于不孕症治疗和精子凝集性避孕药的开发具有潜在意义。
