Huang H, Kochert G
Botany Department, University of Georgia, Athens 30602.
Plant Mol Biol. 1994 Jul;25(4):633-48. doi: 10.1007/BF00029602.
The purpose of this study was to construct a comparative RFLP map of an allotetraploid wild rice species, Oryza latifolia, and to study the relationship between the CCDD genome of O. latifolia and the AA genome of O. sativa. A set of RFLP markers, which had been previously mapped to the AA genome of cultivated rice, were used to construct the comparative map. Fifty-eight F2 progeny, which were derived from a single F1 plant, were used for segregation analysis. The comparative RFLP map contains 149 DNA markers, including 145 genomic DNA markers from cultivated rice, 3 cDNA markers from oat, and one known gene (waxy, from maize). Segregation patterns reflected the allotetraploid ancestry of O. latifolia, and the CC and DD genomes were readily distinguished by most probes tested. There is a high degree of conservation between the CCDD genome of O. latifolia and the AA genome of O. sativa based on our data, but some inversions and translocations were noted.
本研究的目的是构建异源四倍体野生稻物种宽叶野生稻的比较RFLP图谱，并研究宽叶野生稻的CCDD基因组与栽培稻AA基因组之间的关系。一组先前已定位到栽培稻AA基因组的RFLP标记用于构建比较图谱。从单个F1植株衍生而来的58个F2后代用于分离分析。比较RFLP图谱包含149个DNA标记，包括来自栽培稻的145个基因组DNA标记、来自燕麦的3个cDNA标记和一个已知基因（来自玉米的蜡质基因）。分离模式反映了宽叶野生稻的异源四倍体祖先，并且通过大多数测试探针可以很容易地区分CC和DD基因组。基于我们的数据，宽叶野生稻的CCDD基因组与栽培稻的AA基因组之间存在高度保守性，但也注意到了一些倒位和易位。
