Zuany-Amorim C, Cordeiro R S, Vargaftig B B
FIO Cruz, Instituto Oswaldo Cruz, Departamento de Fisiologia Farmacodinâmica, Rio de Janeiro, Brazil.
Eur J Pharmacol. 1994 May 23;257(3):211-6. doi: 10.1016/0014-2999(94)90131-7.
The involvement of leukotrienes in the anaphylactic paw edema was investigated in sensitized boosted or unboosted mice. No difference was noted in the intensity of the antigen-induced paw edema between boosted and unboosted animals. Since the non-steroidal anti-inflammatory agents, indomethacin and aspirin, were inactive, cyclooxygenase products are not involved in the anaphylactic reaction in boosted or unboosted mice. In contrast, the mixed lipoxygenase/cyclooxygenase inhibitor, BW 755C (3-amino-1-m-(trifluoroethyl)phenyl-2-pyrazoline), and the LTD4 (leukotriene D4) receptor antagonist, LY 171883 (1-(2-hydroxy-3-propyl-4-(4-(1H-tetrazol-5-yl)butoxy)phenyl)eth anone, reduced the edema in boosted but not unboosted mice, suggesting the involvement of lipoxygenase metabolites in the allergic edema in boosted mice. Dexamethasone was only effective in inhibiting allergic edema in boosted mice when administered 12 h before provocation, but failed to reduce the edema in unboosted mice. Our results suggest that the booster injection during immunization shifts the anaphylactic mouse paw edema from a leukotriene-independent to a leukotriene-dependent reaction.
在致敏的加强免疫或未加强免疫小鼠中研究了白三烯在过敏性爪水肿中的作用。加强免疫和未加强免疫的动物在抗原诱导的爪水肿强度上没有差异。由于非甾体抗炎药吲哚美辛和阿司匹林无活性，因此环氧化酶产物不参与加强免疫或未加强免疫小鼠的过敏反应。相比之下，混合脂氧合酶/环氧化酶抑制剂BW 755C（3-氨基-1-间-（三氟乙基）苯基-2-吡唑啉）和白三烯D4（LTD4）受体拮抗剂LY 171883（1-（2-羟基-3-丙基-4-（4-（1H-四氮唑-5-基）丁氧基）苯基）乙酮）可减轻加强免疫小鼠而非未加强免疫小鼠的水肿，提示脂氧合酶代谢产物参与加强免疫小鼠的过敏性水肿。地塞米松仅在激发前12小时给药时对抑制加强免疫小鼠的过敏性水肿有效，但对未加强免疫小鼠的水肿无减轻作用。我们的结果表明，免疫期间的加强注射使过敏性小鼠爪水肿从白三烯非依赖性反应转变为白三烯依赖性反应。
