Yamashita A, Yamamoto N, Matsuda J, Koyanagi Y
Department of Microbiology, Tokyo Medical and Dental University, Japan.
Virology. 1994 Oct;204(1):170-9. doi: 10.1006/viro.1994.1521.
The primary targets for infection of HIV-1 are CD4+ T cells and monocyte/macrophage cells. We investigated the genotype heterogeneity of HIV-1 in distinct blood compartments, CD4+ T cells, monocytes, and plasma. Nucleotide sequences of proviral DNA from CD4+ T cells, monocyte cells, and cell-free virion RNA from plasma were determined in three HIV-1 infected individuals. We found that the heterogeneity in proviral DNA from CD4+ T cells was much higher than that found in monocyte cells or in virion RNA from plasma. These data showed that different swarms of HIV-1 exist in individually infected cells and plasma. Furthermore, predicted V3 amino acid sequences in the infected monocytes were very similar to the reported sequence of prototypic macrophage-tropic HIV-1 isolates. Our results show that macrophage-tropic virus is quite homogeneous in monocytes/macrophages and has a common V3 structure in contrast to T-cell-tropic virus in vivo.
HIV-1的主要感染靶细胞是CD4+ T细胞和单核细胞/巨噬细胞。我们研究了HIV-1在不同血液区室（CD4+ T细胞、单核细胞和血浆）中的基因异质性。测定了三名HIV-1感染者CD4+ T细胞原病毒DNA、单核细胞原病毒DNA以及血浆中游离病毒体RNA的核苷酸序列。我们发现，CD4+ T细胞原病毒DNA的异质性远高于单核细胞或血浆中病毒体RNA的异质性。这些数据表明，在个体感染的细胞和血浆中存在不同的HIV-1群体。此外，感染单核细胞中预测的V3氨基酸序列与已报道的原型巨噬细胞嗜性HIV-1分离株序列非常相似。我们的结果表明，与体内T细胞嗜性病毒相比，巨噬细胞嗜性病毒在单核细胞/巨噬细胞中相当一致，并且具有共同的V3结构。
