Cruz H, Pérez C, Wellington E, Castro C, Servín-González L
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, D.F.
Gene. 1994 Jun 24;144(1):141-2. doi: 10.1016/0378-1119(94)90220-8.
An extracellular lipase (Lip)-encoding gene from Streptomyces albus G has been cloned and sequenced. It encodes a Lip with 82% sequence identity to another previously cloned Lip from a Streptomyces species not closely related. These two sequences can be aligned with 33% identity to the sequence of Lip1 from the antarctic psychrotroph Moraxella TA144 [G. Feller et al., Nucleic Acids Res. 18 (1990) 6431]. An alignment of the three sequences revealed amino-acid substitutions which might be responsible for the greater thermal stability of the Streptomyces lipases. The presence of this lip gene family in several members of the Streptomyces genus was also shown.
来自白色链霉菌G的一种细胞外脂肪酶（Lip）编码基因已被克隆和测序。它编码的一种脂肪酶与另一种先前从非近缘链霉菌物种克隆的脂肪酶具有82%的序列同一性。这两个序列与南极嗜冷菌莫拉克斯氏菌TA144的Lip1序列有33%的同一性[G. Feller等人，《核酸研究》18 (1990) 6431]。这三个序列的比对揭示了可能导致链霉菌脂肪酶具有更高热稳定性的氨基酸替换。还证明了该脂肪酶基因家族在链霉菌属的几个成员中存在。
