Oberyé E H, Maurer K, Mager W H, Planta R J
Department of Biochemistry and Molecular Biology, Vrije Universiteit, Amsterdam, The Netherlands.
Biochim Biophys Acta. 1993 May 28;1173(2):233-6. doi: 10.1016/0167-4781(93)90187-i.
By transformation of a Saccharomyces cerevisiae mutant strain conditionally expressing the ABF1-gene, a Kluyveromyces marxianus DNA fragment carrying the gene encoding the ABF1-homologue of this yeast strain (KmABF1) was selected. Comparison of the sequence of the KmABF1 gene with that encoding Saccharomyces cerevisiae ABF1 and the previously isolated ABF1-gene from Kluyveromyces lactis (KlABF1) revealed distinct regions displaying considerable homology and therefore most likely representing sequences encoding essential domains. In addition to the domains putatively involved in DNA binding of the protein factor, two short conserved amino acid sequence elements at the C-termini of the homologous proteins were identified, which are proposed to play a part in their trans-acting functions. This is the first report on the structure of a regulatory protein factor from K. marxianus.
通过对条件性表达ABF1基因的酿酒酵母突变菌株进行转化，筛选出了一个携带该酵母菌株ABF1同源物编码基因（KmABF1）的马克斯克鲁维酵母DNA片段。将KmABF1基因的序列与酿酒酵母ABF1以及先前从乳酸克鲁维酵母中分离出的ABF1基因（KlABF1）的编码序列进行比较，发现了一些明显具有相当同源性的区域，因此很可能代表编码必需结构域的序列。除了推测参与蛋白质因子DNA结合的结构域外，还鉴定出同源蛋白C末端的两个短保守氨基酸序列元件，它们被认为在其反式作用功能中发挥作用。这是关于马克斯克鲁维酵母调控蛋白因子结构的首次报道。
