Chalmers D T, López J F, Vázquez D M, Akil H, Watson S J
Mental Health Research Institute, University of Michigan, Ann Arbor 48109.
Neuropsychopharmacology. 1994 May;10(3):215-22. doi: 10.1038/npp.1994.24.
The effect of dexamethasone, a selective GR agonist, on hippocampal, 5-HT1A receptor mRNA expression and 5-HT1A binding was examined using in situ hybridization histochemistry and in vitro receptor autoradiography. One week after adrenalectomy, both 5-HT1A receptor mRNA expression and 5-HT1A binding were increased throughout the hippocampus. Administration of dexamethasone at the time of adrenalectomy significantly attenuated the increases in 5-HT1A mRNA expression in all hippocampal subfields (p < .05, Fisher Test), although 5-HT1A mRNA levels remained significantly higher than sham levels in all subfields with the exception of CA1. However, 5-HT1A binding levels were responsive to dexamethasone administration only within particular hippocampal subfields, CA1, and dentate gyrus. We conclude that GR occupation negatively regulates 5-HT1A receptor mRNA expression within the hippocampus and that 5-HT1A receptor sites are most sensitive to modulation in those hippocampal subfields expressing higher levels of GR receptors.
使用原位杂交组织化学和体外受体放射自显影技术，研究了选择性糖皮质激素受体（GR）激动剂地塞米松对海马5-羟色胺1A（5-HT1A）受体mRNA表达和5-HT1A结合的影响。肾上腺切除术后一周，整个海马区的5-HT1A受体mRNA表达和5-HT1A结合均增加。在肾上腺切除时给予地塞米松可显著减弱所有海马亚区5-HT1A mRNA表达的增加（p <.05，Fisher检验），尽管除CA1外，所有亚区的5-HT1A mRNA水平仍显著高于假手术组。然而，5-HT1A结合水平仅在特定的海马亚区，即CA1和齿状回，对地塞米松给药有反应。我们得出结论，GR占据负向调节海马内5-HT1A受体mRNA表达，并且5-HT1A受体位点在那些表达较高水平GR受体的海马亚区对调节最为敏感。
