Kawashima H, Mori T, Takekuma K, Hoshika A, Hata M, Nakayama T
Department of Pediatrics, Tokyo Medical College, Japan.
Arch Virol. 1996;141(5):877-84. doi: 10.1007/BF01718162.
We examined the measles H gene using reverse transcriptase-polymerase chain reaction in peripheral mononuclear cells obtained from 4 pediatric and 2 adult patients with autoimmune hepatitis and 12 healthy children who had been infected with measles or vaccinated with an attenuated measles vaccine in the past. All patients were positive for the presence of the gene. Only one healthy control, who had been vaccinated two weeks before the study, was positive, while the other 11 controls were negative for the presence of the gene. The restriction enzyme patterns of the products in the pediatric patients were different from those observed in adults. The sequences of amplified products from pediatric patients coincided with the vaccine strain, whereas those from adults were different from the vaccine strain. The sequence of those from one of two adult patients was similar to those of the isolates in 1990 and later. Our results demonstrated that children with autoimmune hepatitis can have persistence of the vaccine strain in vivo for many years after vaccination.
我们使用逆转录聚合酶链反应对外周血单个核细胞中的麻疹H基因进行了检测，这些外周血单个核细胞取自4例儿童和2例成人自身免疫性肝炎患者，以及12名过去曾感染麻疹或接种过减毒麻疹疫苗的健康儿童。所有患者的该基因检测均呈阳性。只有一名在研究前两周接种过疫苗的健康对照呈阳性，而其他11名对照的该基因检测为阴性。儿童患者中产物的限制性内切酶图谱与成人中观察到的不同。儿童患者扩增产物的序列与疫苗株一致，而成人患者的扩增产物序列与疫苗株不同。两名成人患者中其中一人的序列与1990年及以后分离株的序列相似。我们的结果表明，自身免疫性肝炎患儿在接种疫苗后体内可多年持续存在疫苗株。
