La Thangue N B
Laboratory of Eukaryotic Molecular Genetics, MRC National Institute for Medical Research, London, UK.
Curr Opin Cell Biol. 1994 Jun;6(3):443-50. doi: 10.1016/0955-0674(94)90038-8.
In mammalian cells, DRTF1/E2F is a transcription factor widely believed to integrate cell-cycle progression with the transcription apparatus through its cyclical interactions with important regulators of the cell cycle, such as the retinoblastoma tumour-suppressor gene product, cyclins and cyclin-dependent kinases. Recently, a number of exciting developments have uncovered the heterodimeric nature of DRTF1/E2F by defining two distinct families of proteins, DP and E2F, which comprise its activity; efficient DNA-binding activity arises when a DP protein interacts with an E2F protein. Combinatorial interactions generate a surprising array of sequence-specific heterodimers, a diversity that is probably necessary to enable different cell cycle regulating proteins to integrate their activities with transcription.
在哺乳动物细胞中，DRTF1/E2F是一种转录因子，人们普遍认为它通过与细胞周期的重要调节因子（如视网膜母细胞瘤肿瘤抑制基因产物、细胞周期蛋白和细胞周期蛋白依赖性激酶）进行周期性相互作用，将细胞周期进程与转录装置整合起来。最近，一些令人兴奋的进展通过定义组成其活性的两个不同的蛋白质家族DP和E2F，揭示了DRTF1/E2F的异二聚体性质；当DP蛋白与E2F蛋白相互作用时，会产生有效的DNA结合活性。组合相互作用产生了一系列令人惊讶的序列特异性异二聚体，这种多样性可能是使不同的细胞周期调节蛋白将其活性与转录整合起来所必需的。
