Tieben L M, Berkhout R J, Smits H L, Bouwes Bavinck J N, Vermeer B J, Bruijn J A, Van der Woude F J, Ter Schegget J
Department of Dermatology, University Hospital Leiden, The Netherlands.
Br J Dermatol. 1994 Aug;131(2):226-30. doi: 10.1111/j.1365-2133.1994.tb08496.x.
To evaluate the putative role of human papillomaviruses (HPV) in the development of skin cancer in renal transplant recipients, a series of skin biopsies from premalignant and malignant skin lesions was analysed using the polymerase chain reaction. Four different consensus primer pairs were used. HPV DNA was detected in five of 24 cases of squamous cell carcinoma, in one of three cases of Bowen's disease, in none of four basal cell carcinomas, in two of seven cases of actinic keratosis and in one of five cases of keratoacanthoma. Typing by direct sequencing of the amplified HPV DNA was possible in seven of nine cases, and revealed epidermodysplasia verruciformis (EV)-associated HPV types, or HPV types related to EV-associated types. Hence, HPV DNA could be detected in a significant proportion of (pre)malignant skin tumours in renal transplant recipients. The finding that some of the detected HPV types were as yet uncharacterized EV-related types, suggests that HPV DNA could be present in a higher percentage of lesions, and might be detected with refinement of the techniques.
为评估人乳头瘤病毒（HPV）在肾移植受者皮肤癌发生中的假定作用，我们使用聚合酶链反应对一系列来自癌前和恶性皮肤病变的皮肤活检样本进行了分析。使用了四对不同的共有引物。在24例鳞状细胞癌中有5例检测到HPV DNA，3例鲍温病中有1例检测到，4例基底细胞癌中均未检测到，7例光化性角化病中有2例检测到，5例角化棘皮瘤中有1例检测到。在9例中的7例中，通过对扩增的HPV DNA进行直接测序得以分型，结果显示为疣状表皮发育不良（EV）相关的HPV类型，或与EV相关类型有关的HPV类型。因此，在肾移植受者的相当一部分（癌前和）恶性皮肤肿瘤中可检测到HPV DNA。所检测到的一些HPV类型为尚未明确的与EV相关的类型，这一发现表明HPV DNA可能在更高比例的病变中存在，并且随着技术的改进可能会被检测到。
