利用电子顺磁共振监测膜中螺旋肽之间的碰撞：表明在没有膜电位的情况下短杆菌肽A是单体的证据。
Collisions between helical peptides in membranes monitored using electron paramagnetic resonance: evidence that alamethicin is monomeric in the absence of a membrane potential.
作者信息
Barranger-Mathys M, Cafiso D S
机构信息
Department of Chemistry, University of Virginia, Charlottesville 22901.
出版信息
Biophys J. 1994 Jul;67(1):172-6. doi: 10.1016/S0006-3495(94)80466-7.
Abstract
Alamethicin is a 20-amino-acid peptide that produces a voltage-dependent conductance in membranes. We investigated the state of aggregation of alamethicin in egg phosphatidylcholine and dioleoylphosphatidylcholine membranes by examining the EPR spectra obtained from an active analog of this peptide that is spin-labeled at its C-terminus. The dependence of both the linewidth and signal intensity as a function of peptide concentration exhibit exchange broadening as the peptide concentration is increased; however, the exchange rates are linear with peptide concentration as is expected for the simple diffusion of monomers. In addition, the spin-exchange rates obtained from the linebroadening are consistent with collisional rates that are predicted from free Brownian diffusion. The results provide strong evidence that in the absence of a membrane potential, alamethicin is largely monomeric in these membranes.
摘要
阿拉霉素是一种由20个氨基酸组成的肽，可在膜中产生电压依赖性电导。我们通过检查从该肽的活性类似物（其C端进行了自旋标记）获得的电子顺磁共振（EPR）光谱，研究了阿拉霉素在鸡蛋磷脂酰胆碱和二油酰磷脂酰胆碱膜中的聚集状态。随着肽浓度的增加，线宽和信号强度对肽浓度的依赖性均表现出交换加宽；然而，交换速率与肽浓度呈线性关系，这与单体的简单扩散预期一致。此外，从线宽加宽获得的自旋交换速率与自由布朗扩散预测的碰撞速率一致。结果提供了有力证据，表明在没有膜电位的情况下，阿拉霉素在这些膜中主要以单体形式存在。
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本文引用的文献
1
Lipid-alamethicin interactions influence alamethicin orientation.脂质与拉米夫定相互作用影响拉米夫定的取向。
Biophys J. 1991 Nov;60(5):1079-87. doi: 10.1016/S0006-3495(91)82144-0.
2
CHROMATOGRAPHICALLY HOMOGENEOUS LECITHIN FROM EGG PHOSPHOLIPIDS.源自鸡蛋磷脂的色谱纯卵磷脂
J Am Oil Chem Soc. 1965 Jan;42:53-6. doi: 10.1007/BF02558256.
3
Temperature dependence of the interaction of alamethicin helices in membranes.膜中短杆菌肽螺旋相互作用的温度依赖性。
Biochemistry. 1993 Sep 21;32(37):9819-25. doi: 10.1021/bi00088a037.
4
Alamethicin and related peptaibols--model ion channels.短杆菌肽A及相关肽抗生素——离子通道模型
Eur Biophys J. 1993;22(2):105-24. doi: 10.1007/BF00196915.
5
Structure of micelle-associated alamethicin from 1H NMR. Evidence for conformational heterogeneity in a voltage-gated peptide.基于核磁共振氢谱的与胶束相关的阿拉米辛结构。一种电压门控肽中构象异质性的证据。
Biochemistry. 1994 Apr 5;33(13):4036-45. doi: 10.1021/bi00179a032.
6
Alamethicin-induced changes in lipid bilayer morphology.短杆菌肽诱导的脂质双层形态变化。
Biochim Biophys Acta. 1982 Feb 8;685(1):51-60. doi: 10.1016/0005-2736(82)90034-7.
7
Alamethicin. A rich model for channel behavior.阿拉米辛。通道行为的丰富模型。
Biophys J. 1984 Jan;45(1):233-47. doi: 10.1016/S0006-3495(84)84151-X.
8
Studies of the crystalline-liquid crystalline phase transition of lipid model membranes. II. Analysis of electron spin resonance spectra of steroid labels incorporated into lipid membranes.脂质模型膜的晶态-液晶态相变研究。II. 掺入脂质膜的类固醇标记物的电子自旋共振光谱分析。
J Am Chem Soc. 1972 Jun 28;94(13):4492-8. doi: 10.1021/ja00768a014.
9
Lipid dependence of peptide-membrane interactions. Bilayer affinity and aggregation of the peptide alamethicin.肽与膜相互作用的脂质依赖性。肽缬氨霉素的双层亲和力和聚集作用。
FEBS Lett. 1989 Jul 3;250(2):556-60. doi: 10.1016/0014-5793(89)80795-1.
10
Location and dynamics of alamethicin in unilamellar vesicles and thylakoids as model systems. A spin label study.
Biochim Biophys Acta. 1989 Nov 17;986(1):47-60. doi: 10.1016/0005-2736(89)90271-x.
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