Kühner M, Tampé R, Sackmann E
Physik Department, Technische Universität München, Garching/München, Germany.
Biophys J. 1994 Jul;67(1):217-26. doi: 10.1016/S0006-3495(94)80472-2.
We report the deposition of lipid monolayers and bilayers on polyacrylamide films deposited by radical chain reaction onto solid substrates in aqueous solutions. Polymer films of various degrees of monomer density and cross-linking are prepared. Lateral diffusion and fluorescent probe permeation measurements yield insight into the continuity of the lipid layers and show that monolayers exposed to air are much less sensitive towards polymer heterogeneities than bilayers below water, which is explained in terms of the wetting laws. The diffusion studies of lipid and lipopeptide probes yield absolute values of the frictional coefficients between the lipid layer and the polymer films and allow one to estimate the surface viscosity of the polymer film. The potential applications of supported membranes on soft thin polymer films for the preparation of biofunctionalized surfaces or biocompatible receptive surfaces for biosensors are discussed.
我们报道了在水溶液中通过自由基链反应沉积在固体基质上的聚丙烯酰胺薄膜上脂质单层和双层的沉积情况。制备了具有不同单体密度和交联度的聚合物薄膜。横向扩散和荧光探针渗透测量有助于深入了解脂质层的连续性，结果表明暴露于空气中的单层对聚合物不均匀性的敏感度远低于水下的双层，这可以根据润湿定律来解释。脂质和脂肽探针的扩散研究得出了脂质层与聚合物薄膜之间摩擦系数的绝对值，并能够估算聚合物薄膜的表面粘度。还讨论了在柔软的薄聚合物薄膜上的支撑膜在制备生物功能化表面或用于生物传感器的生物相容性接受表面方面的潜在应用。
